1gqu
From Proteopedia
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| - | [[Image:1gqu.gif|left|200px]]<br /><applet load="1gqu" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1gqu, resolution 2.5Å" /> | ||
| - | '''CRYSTAL STRUCTURE OF AN ALTERNATING A-T OLIGONUCLEOTIDE FRAGMENT WITH HOOGSTEEN BASE PAIRING'''<br /> | ||
| - | == | + | ==Crystal structure of an alternating A-T oligonucleotide fragment with Hoogsteen base pairing== |
| + | <StructureSection load='1gqu' size='340' side='right'caption='[[1gqu]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1gqu]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GQU FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gqu OCA], [https://pdbe.org/1gqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gqu RCSB], [https://www.ebi.ac.uk/pdbsum/1gqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gqu ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
We report here an alternative double-helical structure of the DNA molecule. It has been found in the d(ATA(Br)UAT) and d(ATATAT) sequences by single-crystal x-ray crystallography. This sequence is found not only in TATA boxes, but also in other regulatory regions of DNA. Bases of the two antiparallel strands form Hoogsteen pairs, with adenines in the syn conformation. The structure is related neither to those found in triple helices nor to parallel DNA duplexes. Its conformational parameters are very similar to those of duplex DNA in the B form. Both forms may coexist under physiological conditions, although the Hoogsteen pairing greatly influences the recognition sites on DNA. Our results demonstrate that an alternative to the classical B-DNA double helix is possible. | We report here an alternative double-helical structure of the DNA molecule. It has been found in the d(ATA(Br)UAT) and d(ATATAT) sequences by single-crystal x-ray crystallography. This sequence is found not only in TATA boxes, but also in other regulatory regions of DNA. Bases of the two antiparallel strands form Hoogsteen pairs, with adenines in the syn conformation. The structure is related neither to those found in triple helices nor to parallel DNA duplexes. Its conformational parameters are very similar to those of duplex DNA in the B form. Both forms may coexist under physiological conditions, although the Hoogsteen pairing greatly influences the recognition sites on DNA. Our results demonstrate that an alternative to the classical B-DNA double helix is possible. | ||
| - | + | Crystal structure of an antiparallel DNA fragment with Hoogsteen base pairing.,Abrescia NG, Thompson A, Huynh-Dinh T, Subirana JA Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2806-11. PMID:11880632<ref>PMID:11880632</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 1gqu" style="background-color:#fffaf0;"></div> |
| - | [[Category: Abrescia | + | == References == |
| - | [[Category: Huynh-Dinh | + | <references/> |
| - | [[Category: Subirana | + | __TOC__ |
| - | [[Category: Thompson | + | </StructureSection> |
| - | + | [[Category: Large Structures]] | |
| - | + | [[Category: Abrescia NGA]] | |
| - | + | [[Category: Huynh-Dinh T]] | |
| - | + | [[Category: Subirana JA]] | |
| + | [[Category: Thompson A]] | ||
Current revision
Crystal structure of an alternating A-T oligonucleotide fragment with Hoogsteen base pairing
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