1ujz

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[[Image:1ujz.png|left|200px]]
 
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{{STRUCTURE_1ujz| PDB=1ujz | SCENE= }}
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==Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein==
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<StructureSection load='1ujz' size='340' side='right'caption='[[1ujz]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ujz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UJZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ujz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ujz OCA], [https://pdbe.org/1ujz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ujz RCSB], [https://www.ebi.ac.uk/pdbsum/1ujz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ujz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uj/1ujz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ujz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We developed a 'computational second-site suppressor' strategy to redesign specificity at a protein-protein interface and applied it to create new specifically interacting DNase-inhibitor protein pairs. We demonstrate that the designed switch in specificity holds in in vitro binding and functional assays. We also show that the designed interfaces are specific in the natural functional context in living cells, and present the first high-resolution X-ray crystallographic analysis of a computer-redesigned functional protein-protein interface with altered specificity. The approach should be applicable to the design of interacting protein pairs with novel specificities for delineating and re-engineering protein interaction networks in living cells.
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===Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein===
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Computational redesign of protein-protein interaction specificity.,Kortemme T, Joachimiak LA, Bullock AN, Schuler AD, Stoddard BL, Baker D Nat Struct Mol Biol. 2004 Apr;11(4):371-9. Epub 2004 Mar 21. PMID:15034550<ref>PMID:15034550</ref>
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{{ABSTRACT_PUBMED_15034550}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1ujz" style="background-color:#fffaf0;"></div>
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[[1ujz]] is a 2 chain structure of [[Colicin]] and [[Colicin Immunity Protein]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJZ OCA].
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==See Also==
==See Also==
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*[[Colicin|Colicin]]
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*[[Colicin 3D structures|Colicin 3D structures]]
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*[[Colicin Immunity Protein|Colicin Immunity Protein]]
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*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015034550</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Baker, D.]]
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[[Category: Large Structures]]
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[[Category: Bullock, A N.]]
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[[Category: Baker D]]
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[[Category: Joachimiak, L A.]]
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[[Category: Bullock AN]]
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[[Category: Kortemme, T.]]
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[[Category: Joachimiak LA]]
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[[Category: Schuler, A D.]]
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[[Category: Kortemme T]]
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[[Category: Stoddard, B L.]]
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[[Category: Schuler AD]]
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[[Category: Computational design]]
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[[Category: Stoddard BL]]
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[[Category: Immune system]]
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[[Category: Molecular recognition]]
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[[Category: Protein complex]]
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[[Category: Redesigned protein-protein interface]]
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[[Category: Specificity]]
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Current revision

Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein

PDB ID 1ujz

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