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2cia

From Proteopedia

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[[Image:2cia.gif|left|200px]]<br />
 
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<applet load="2cia" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cia, resolution 1.45&Aring;" />
 
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'''HUMAN NCK2 SH2-DOMAIN IN COMPLEX WITH A DECAPHOSPHOPEPTIDE FROM TRANSLOCATED INTIMIN RECEPTOR (TIR) OF EPEC'''<br />
 
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==Overview==
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==human nck2 sh2-domain in complex with a decaphosphopeptide from translocated intimin receptor (tir) of epec==
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Nck proteins are essential Src homology (SH) 2 and SH3 domain-bearing, adapters that modulate actin cytoskeleton dynamics by linking proline-rich, effector molecules to tyrosine kinases or phosphorylated signaling, intermediates. Two mammalian pathogens, enteropathogenic Escherichia coli, and vaccinia virus, exploit Nck as part of their infection strategy., Conflicting data indicate potential differences in the recognition, specificities of the SH2 domains of the isoproteins Nck1 (Nckalpha) and, Nck2 (Nckbeta and Grb4). We have characterized the binding specificities, of both SH2 domains and find them to be essentially indistinguishable., Crystal structures of both domains in complex with phosphopeptides derived, from the enteropathogenic E. coli protein Tir concur in identifying highly, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?16636066 (full description)]]
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<StructureSection load='2cia' size='340' side='right'caption='[[2cia]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2cia]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CIA FirstGlance]. <br>
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2CIA is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with ACE, MPD and IPA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CIA OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cia OCA], [https://pdbe.org/2cia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cia RCSB], [https://www.ebi.ac.uk/pdbsum/2cia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cia ProSAT]</span></td></tr>
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The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains., Frese S, Schubert WD, Findeis AC, Marquardt T, Roske YS, Stradal TE, Heinz DW, J Biol Chem. 2006 Jun 30;281(26):18236-45. Epub 2006 Apr 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16636066 16636066]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NCK2_HUMAN NCK2_HUMAN] Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.<ref>PMID:10026169</ref> <ref>PMID:16835242</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/2cia_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cia ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Findeis, A.C.]]
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[[Category: Findeis AC]]
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[[Category: Frese, S.]]
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[[Category: Frese S]]
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[[Category: Heinz, D.W.]]
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[[Category: Heinz DW]]
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[[Category: Marquardt, T.]]
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[[Category: Marquardt T]]
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[[Category: Roske, Y.S.]]
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[[Category: Roske YS]]
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[[Category: Schubert, W.D.]]
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[[Category: Schubert W-D]]
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[[Category: Stradal, T.E.B.]]
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[[Category: Stradal TEB]]
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[[Category: ACE]]
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[[Category: IPA]]
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[[Category: MPD]]
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[[Category: binding specificity]]
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[[Category: phosphorylation]]
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[[Category: sh2-domain]]
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[[Category: sh3 domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:10:38 2007''
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Current revision

human nck2 sh2-domain in complex with a decaphosphopeptide from translocated intimin receptor (tir) of epec

PDB ID 2cia

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