1jzx
From Proteopedia
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| - | [[Image:1jzx.png|left|200px]] | ||
| - | + | ==Structural Basis for the Interaction of Antibiotics with the Peptidyl Transferase Center in Eubacteria== | |
| - | + | <StructureSection load='1jzx' size='340' side='right'caption='[[1jzx]], [[Resolution|resolution]] 3.10Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1jzx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JZX FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> | |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLY:CLINDAMYCIN'>CLY</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |
| - | == | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jzx OCA], [https://pdbe.org/1jzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jzx RCSB], [https://www.ebi.ac.uk/pdbsum/1jzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jzx ProSAT]</span></td></tr> |
| - | [[1jzx | + | </table> |
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RL22_DEIRA RL22_DEIRA] This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity).[HAMAP-Rule:MF_01331_B] The globular domain of the protein is located by the polypeptide exit tunnel on the outside of the subunit while an extended beta-hairpin forms part of the wall of the tunnel. Forms a pair of "tweezers" with L32 that hold together two different domains of the 23S rRNA. Interacts with the tunnel-blocking modified macrolide azithromycin. Upon binding of the macrolide troleadomycin to the ribosome, the tip of the beta-hairpin is displaced, which severely restricts the tunnel. This and experiments in E.coli have led to the suggestion that it is part of the gating mechanism involved in translation arrest in the absence of the protein export system.[HAMAP-Rule:MF_01331_B] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jz/1jzx_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jzx ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
==See Also== | ==See Also== | ||
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*[[Ribosomal protein L32|Ribosomal protein L32]] | *[[Ribosomal protein L32|Ribosomal protein L32]] | ||
*[[Ribosomal protein L4|Ribosomal protein L4]] | *[[Ribosomal protein L4|Ribosomal protein L4]] | ||
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
[[Category: Deinococcus radiodurans]] | [[Category: Deinococcus radiodurans]] | ||
| - | [[Category: Albrecht | + | [[Category: Large Structures]] |
| - | [[Category: Bashan | + | [[Category: Albrecht R]] |
| - | [[Category: Franceschi | + | [[Category: Bashan A]] |
| - | [[Category: Harms | + | [[Category: Franceschi F]] |
| - | [[Category: Schluenzen | + | [[Category: Harms J]] |
| - | [[Category: Tocilj | + | [[Category: Schluenzen F]] |
| - | [[Category: Yonath | + | [[Category: Tocilj A]] |
| - | [[Category: Zarivach | + | [[Category: Yonath A]] |
| - | + | [[Category: Zarivach R]] | |
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Current revision
Structural Basis for the Interaction of Antibiotics with the Peptidyl Transferase Center in Eubacteria
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