2x7n
From Proteopedia
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| - | [[Image:2x7n.png|left|200px]] | ||
| - | + | ==Mechanism of eIF6s anti-association activity== | |
| + | <SX load='2x7n' size='340' side='right' viewer='molstar' caption='[[2x7n]], [[Resolution|resolution]] 11.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2x7n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X7N FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.8Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x7n OCA], [https://pdbe.org/2x7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x7n RCSB], [https://www.ebi.ac.uk/pdbsum/2x7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x7n ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RL23A_YEAST RL23A_YEAST] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/2x7n_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x7n ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | During the process of ribosomal assembly, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from Saccharomyces cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in Escherichia coli). Based on our structural data, we propose that the mechanism of the anti-association activity of eIF6 is based on steric hindrance of intersubunit bridge formation around the dynamic bridge B6. | ||
| - | + | Mechanism of eIF6-mediated inhibition of ribosomal subunit joining.,Gartmann M, Blau M, Armache JP, Mielke T, Topf M, Beckmann R J Biol Chem. 2010 May 14;285(20):14848-51. Epub 2010 Mar 31. PMID:20356839<ref>PMID:20356839</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2x7n" style="background-color:#fffaf0;"></div> | |
| - | + | ||
==See Also== | ==See Also== | ||
| + | *[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]] | ||
*[[Ribosomal protein L23|Ribosomal protein L23]] | *[[Ribosomal protein L23|Ribosomal protein L23]] | ||
| - | + | *[[Ribosomal protein L24|Ribosomal protein L24]] | |
| - | == | + | == References == |
| - | < | + | <references/> |
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Armache | + | [[Category: Armache J-P]] |
| - | [[Category: Beckmann | + | [[Category: Beckmann R]] |
| - | [[Category: Blau | + | [[Category: Blau M]] |
| - | [[Category: Gartmann | + | [[Category: Gartmann M]] |
| - | [[Category: Mielke | + | [[Category: Mielke T]] |
| - | [[Category: Topf | + | [[Category: Topf M]] |
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Current revision
Mechanism of eIF6s anti-association activity
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