1hdp

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[[Image:1hdp.gif|left|200px]]<br /><applet load="1hdp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hdp" />
 
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'''SOLUTION STRUCTURE OF A POU-SPECIFIC HOMEODOMAIN: 3D-NMR STUDIES OF HUMAN B-CELL TRANSCRIPTION FACTOR OCT-2'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF A POU-SPECIFIC HOMEODOMAIN: 3D-NMR STUDIES OF HUMAN B-CELL TRANSCRIPTION FACTOR OCT-2==
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The POU DNA-binding motif defines a conserved family of eukaryotic transcription factors involved in regulation of gene expression. This bipartite motif consists of an N-terminal POU-specific domain (POUs), a flexible linker, and a C-terminal POU-specific homeodomain (POUHD). Here we describe the solution structure of a POU-specific homeodomain. An NMR model is obtained from Oct-2, a human B-cell specific transcription factor which participates in the regulation of immunoglobulin genes. A fragment of Oct-2 containing POUHD and an adjoining linker was expressed in Escherichia coli and characterized by three-dimensional nuclear magnetic resonance (3D-NMR) spectroscopy. Complete 1H and 15N resonance assignment of the POUHD moiety is presented. The POUHD solution structure, as calculated by distance geometry and simulated annealing (DG/SA), is similar to that of canonical homeodomains. A salient difference between solution and crystal structures is observed in the C-terminal segment of alpha-helix 3 (the HTH recognition helix), which is not well ordered in solution. Because this segment presumably folds upon specific DNA binding, its flexibility in solution may reduce the intrinsic DNA affinity of POUHD in the absence of POUs.
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<StructureSection load='1hdp' size='340' side='right'caption='[[1hdp]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1hdp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HDP FirstGlance]. <br>
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1HDP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HDP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hdp OCA], [https://pdbe.org/1hdp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hdp RCSB], [https://www.ebi.ac.uk/pdbsum/1hdp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hdp ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structure of a POU-specific homeodomain: 3D-NMR studies of human B-cell transcription factor Oct-2., Sivaraja M, Botfield MC, Mueller M, Jancso A, Weiss MA, Biochemistry. 1994 Aug 23;33(33):9845-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7914745 7914745]
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== Function ==
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[https://www.uniprot.org/uniprot/PO2F2_HUMAN PO2F2_HUMAN] Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.<ref>PMID:2904654</ref> <ref>PMID:2328728</ref> <ref>PMID:2901913</ref> <ref>PMID:1739980</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hd/1hdp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hdp ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Botfield, M C.]]
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[[Category: Botfield MC]]
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[[Category: Jancso, A.]]
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[[Category: Jancso A]]
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[[Category: Mueller, M.]]
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[[Category: Mueller M]]
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[[Category: Sivaraja, M.]]
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[[Category: Sivaraja M]]
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[[Category: Weiss, M A.]]
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[[Category: Weiss MA]]
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[[Category: dna-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:00:23 2008''
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Current revision

SOLUTION STRUCTURE OF A POU-SPECIFIC HOMEODOMAIN: 3D-NMR STUDIES OF HUMAN B-CELL TRANSCRIPTION FACTOR OCT-2

PDB ID 1hdp

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