1gpt

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[[Image:1gpt.png|left|200px]]
 
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{{STRUCTURE_1gpt| PDB=1gpt | SCENE= }}
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==SOLUTION STRUCTURE OF GAMMA 1-H AND GAMMA 1-P THIONINS FROM BARLEY AND WHEAT ENDOSPERM DETERMINED BY 1H-NMR: A STRUCTURAL MOTIF COMMON TO TOXIC ARTHROPOD PROTEINS==
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<StructureSection load='1gpt' size='340' side='right'caption='[[1gpt]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GPT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 8 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpt OCA], [https://pdbe.org/1gpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gpt RCSB], [https://www.ebi.ac.uk/pdbsum/1gpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DEF1_HORVU DEF1_HORVU] Inhibits protein translation in cell-free systems.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gp/1gpt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gpt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The complete assignment of the proton NMR spectra of the homologous gamma 1-hordothionin and gamma 1-purothionin (47 amino acids, 4 disulfide bridges) from barley and wheat, respectively, has been performed by two-dimensional sequence-specific methods. A total of 299 proton-proton distance constraints for gamma 1-H and 285 for gamma 1-P derived from NOESY spectra have been used to calculate the three-dimensional solution structures. Initial structures have been generated by distance geometry methods and further refined by dynamical simulated annealing calculations. Both proteins show identical secondary and tertiary structure with a well-defined triple-stranded antiparallel beta-sheet (residues 1-6, 31-34, and 39-47), an alpha-helix (residues 16-28), and the corresponding connecting loops. Three disulfide bridges are located in the hydrophobic core holding together the alpha-helix and the beta-sheet and forming a cysteine-stabilized alpha-helical (CSH) motif. Moreover, a clustering of positive charges is observed on the face of the beta-sheet opposite to the helix. The three-dimensional structures of the gamma-thionins differ remarkably from plant alpha- and beta-thionins and crambin. However, they show a higher structural analogy with scorpion toxins and insect defensins which also present the CSH motif.
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===SOLUTION STRUCTURE OF GAMMA 1-H AND GAMMA 1-P THIONINS FROM BARLEY AND WHEAT ENDOSPERM DETERMINED BY 1H-NMR: A STRUCTURAL MOTIF COMMON TO TOXIC ARTHROPOD PROTEINS===
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Solution structure of gamma 1-H and gamma 1-P thionins from barley and wheat endosperm determined by 1H-NMR: a structural motif common to toxic arthropod proteins.,Bruix M, Jimenez MA, Santoro J, Gonzalez C, Colilla FJ, Mendez E, Rico M Biochemistry. 1993 Jan 19;32(2):715-24. PMID:8380707<ref>PMID:8380707</ref>
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{{ABSTRACT_PUBMED_8380707}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1gpt" style="background-color:#fffaf0;"></div>
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[[1gpt]] is a 1 chain structure of [[Plant defensin]] with sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPT OCA].
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==See Also==
==See Also==
*[[Plant defensin|Plant defensin]]
*[[Plant defensin|Plant defensin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:008380707</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
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[[Category: Bruix, M.]]
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[[Category: Large Structures]]
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[[Category: Colilla, F J.]]
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[[Category: Bruix M]]
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[[Category: Gonzalez, C.]]
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[[Category: Colilla FJ]]
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[[Category: Jimenez, M A.]]
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[[Category: Gonzalez C]]
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[[Category: Mendez, E.]]
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[[Category: Jimenez MA]]
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[[Category: Rico, M.]]
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[[Category: Mendez E]]
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[[Category: Santoro, J.]]
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[[Category: Rico M]]
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[[Category: Plant toxin]]
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[[Category: Santoro J]]

Current revision

SOLUTION STRUCTURE OF GAMMA 1-H AND GAMMA 1-P THIONINS FROM BARLEY AND WHEAT ENDOSPERM DETERMINED BY 1H-NMR: A STRUCTURAL MOTIF COMMON TO TOXIC ARTHROPOD PROTEINS

PDB ID 1gpt

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