2bof

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[[Image:2bof.png|left|200px]]
 
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{{STRUCTURE_2bof| PDB=2bof | SCENE= }}
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==Catalytic domain of endo-1,4-glucanase Cel6A mutant Y73S from Thermobifida fusca in complex with cellotetrose==
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<StructureSection load='2bof' size='340' side='right'caption='[[2bof]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bof]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BOF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bof OCA], [https://pdbe.org/2bof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bof RCSB], [https://www.ebi.ac.uk/pdbsum/2bof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bof ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GUN2_THEFU GUN2_THEFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/2bof_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bof ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(2)-S-Glc(2)), has been determined to 1.5 A resolution. The glycosyl unit in subsite -1 was sterically hindered by Tyr73 and forced into a distorted (2)S(o) conformation. In the enzyme where Tyr73 was mutated to a serine residue, the hindrance was removed and the glycosyl unit in subsite -1 had a relaxed (4)C(1) chair conformation. The relaxed conformation was seen in two complex structures of the mutated enzyme, with cellotetrose (Glc(4)) at 1.64 A and Glc(2)-S-Glc(2) at 1.04 A resolution.
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===CATALYTIC DOMAIN OF ENDO-1,4-GLUCANASE CEL6A MUTANT Y73S FROM THERMOBIFIDA FUSCA IN COMPLEX WITH CELLOTETROSE===
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Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion.,Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA Biochemistry. 2005 Oct 4;44(39):12915-22. PMID:16185060<ref>PMID:16185060</ref>
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{{ABSTRACT_PUBMED_16185060}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2bof" style="background-color:#fffaf0;"></div>
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[[2bof]] is a 1 chain structure of [[Glucanase]] with sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BOF OCA].
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==See Also==
==See Also==
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*[[Glucanase|Glucanase]]
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016185060</ref><references group="xtra"/>
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__TOC__
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[[Category: Cellulase]]
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</StructureSection>
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[[Category: Thermobifida fusca]]
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[[Category: Large Structures]]
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[[Category: Bergfors, T.]]
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[[Category: Driguez, H.]]
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[[Category: Dultz, E.]]
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[[Category: Irwin, D C.]]
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[[Category: Jones, T A.]]
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[[Category: Larsson, A M.]]
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[[Category: Roos, A.]]
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[[Category: Wilson, D B.]]
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[[Category: Endoglucanase]]
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[[Category: Glycoside hydrolase family 6]]
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[[Category: Hydrolase]]
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[[Category: Methyl cellobiosyl-4-thio-beta- cellobioside]]
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[[Category: Thermobifida fusca]]
[[Category: Thermobifida fusca]]
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[[Category: Tim a/b fold]]
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[[Category: Bergfors T]]
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[[Category: Driguez H]]
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[[Category: Dultz E]]
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[[Category: Irwin DC]]
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[[Category: Jones TA]]
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[[Category: Larsson AM]]
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[[Category: Roos A]]
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[[Category: Wilson DB]]

Current revision

Catalytic domain of endo-1,4-glucanase Cel6A mutant Y73S from Thermobifida fusca in complex with cellotetrose

PDB ID 2bof

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