1cy9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1cy9" [edit=sysop:move=sysop])
Current revision (06:46, 7 February 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1cy9.png|left|200px]]
 
-
{{STRUCTURE_1cy9| PDB=1cy9 | SCENE= }}
+
==CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. MONOCLINIC FORM==
-
 
+
<StructureSection load='1cy9' size='340' side='right'caption='[[1cy9]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
===CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. MONOCLINIC FORM===
+
== Structural highlights ==
-
 
+
<table><tr><td colspan='2'>[[1cy9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CY9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CY9 FirstGlance]. <br>
-
{{ABSTRACT_PUBMED_10504724}}
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cy9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cy9 OCA], [https://pdbe.org/1cy9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cy9 RCSB], [https://www.ebi.ac.uk/pdbsum/1cy9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cy9 ProSAT]</span></td></tr>
-
==About this Structure==
+
</table>
-
[[1cy9]] is a 2 chain structure of [[Topoisomerase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CY9 OCA].
+
== Function ==
 +
[https://www.uniprot.org/uniprot/TOP1_ECOLI TOP1_ECOLI] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.<ref>PMID:9497321</ref> <ref>PMID:10681504</ref> <ref>PMID:21482796</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cy/1cy9_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cy9 ConSurf].
 +
<div style="clear:both"></div>
==See Also==
==See Also==
-
*[[Topoisomerase|Topoisomerase]]
+
*[[Topoisomerase 3D structures|Topoisomerase 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:010504724</ref><references group="xtra"/>
+
__TOC__
-
[[Category: DNA topoisomerase]]
+
</StructureSection>
-
[[Category: Escherichia coli]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Feinberg, H.]]
+
[[Category: Large Structures]]
-
[[Category: Lima, C.]]
+
[[Category: Feinberg H]]
-
[[Category: Mondragon, A.]]
+
[[Category: Lima C]]
-
[[Category: Decatenating enzyme]]
+
[[Category: Mondragon A]]
-
[[Category: Dna topoisomerase]]
+
-
[[Category: Isomerase]]
+

Current revision

CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. MONOCLINIC FORM

PDB ID 1cy9

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1cy9"
Personal tools