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1hpw

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[[Image:1hpw.gif|left|200px]]<br /><applet load="1hpw" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hpw" />
 
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'''STRUCTURE OF A PILIN MONOMER FROM PSEUDOMONAS AERUGINOSA: IMPLICATIONS FOR THE ASSEMBLY OF PILI.'''<br />
 
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==Overview==
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==STRUCTURE OF A PILIN MONOMER FROM PSEUDOMONAS AERUGINOSA: IMPLICATIONS FOR THE ASSEMBLY OF PILI.==
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Type IV pilin monomers assemble to form fibers called pili that are required for a variety of bacterial functions. Pilin monomers oligomerize due to the interaction of part of their hydrophobic N-terminal alpha-helix. Engineering of a truncated pilin from Pseudomonas aeruginosa strain K122-4, where the first 28 residues are removed from the N terminus, yields a soluble, monomeric protein. This truncated pilin is shown to bind to its receptor and to decrease morbidity and mortality in mice upon administration 15 min before challenge with a heterologous strain of Pseudomonas. The structure of this truncated pilin reveals an alpha-helix at the N terminus that lies across a 4-stranded antiparallel beta-sheet. A model for a pilus is proposed that takes into account both electrostatic and hydrophobic interactions of pilin subunits as well as previously published x-ray fiber diffraction data. Our model indicates that DNA or RNA cannot pass through the center of the pilus, however, the possibility exists for small organic molecules to pass through indicating a potential mechanism for signal transduction.
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<StructureSection load='1hpw' size='340' side='right'caption='[[1hpw]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hpw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HPW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hpw OCA], [https://pdbe.org/1hpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hpw RCSB], [https://www.ebi.ac.uk/pdbsum/1hpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hpw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FMP1_PSEAI FMP1_PSEAI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hp/1hpw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hpw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1HPW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HPW OCA].
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*[[Pilin 3D structures|Pilin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of a pilin monomer from Pseudomonas aeruginosa: implications for the assembly of pili., Keizer DW, Slupsky CM, Kalisiak M, Campbell AP, Crump MP, Sastry PA, Hazes B, Irvin RT, Sykes BD, J Biol Chem. 2001 Jun 29;276(26):24186-93. Epub 2001 Apr 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11294863 11294863]
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Campbell AP]]
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[[Category: Campbell, A P.]]
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[[Category: Irvin RT]]
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[[Category: Irvin, R T.]]
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[[Category: Keizer DW]]
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[[Category: Keizer, D W.]]
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[[Category: Slupsky CM]]
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[[Category: Slupsky, C M.]]
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[[Category: Sykes BD]]
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[[Category: Sykes, B D.]]
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[[Category: fimbria]]
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[[Category: methylation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:03:37 2008''
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Current revision

STRUCTURE OF A PILIN MONOMER FROM PSEUDOMONAS AERUGINOSA: IMPLICATIONS FOR THE ASSEMBLY OF PILI.

PDB ID 1hpw

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