1h4i

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[[Image:1h4i.png|left|200px]]
 
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{{STRUCTURE_1h4i| PDB=1h4i | SCENE= }}
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==Methylobacterium extorquens methanol dehydrogenase==
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<StructureSection load='1h4i' size='340' side='right'caption='[[1h4i]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1h4i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens Methylorubrum extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4i OCA], [https://pdbe.org/1h4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4i RCSB], [https://www.ebi.ac.uk/pdbsum/1h4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4i ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHM1_METEA DHM1_METEA] Catalyzes the oxidation of primary alcohols including methanol.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h4i ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Methanol dehydrogenase (MDH) is a bacterial periplasmic quinoprotein; it has pyrrolo-quinoline quinone (PQQ) as its prosthetic group, requires Ca2+ for activity and uses cytochrome cL as its electron acceptor. Low-resolution structures of MDH have already been determined. RESULTS: The structure of the alpha 2 beta 2 tetramer of MDH from Methylobacterium extorquens has now been determined at 1.94 A with an R-factor of 19.85%. CONCLUSIONS: The alpha-subunit of MDH has an eight-fold radial symmetry, with its eight beta-sheets stabilized by a novel tryptophan docking motif. The PQQ in the active site is held in place by a coplanar tryptophan and by a novel disulphide ring formed between adjacent cysteines which are bonded by an unusual non-planar trans peptide bond. One of the carbonyl oxygens of PQQ is bonded to the Ca2+, probably facilitating attack on the substrate, and the other carbonyl oxygen is out of the plane of the ring, confirming the presence of the predicted free-radical semiquinone form of the prosthetic group.
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===METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE===
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The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A.,Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C Structure. 1995 Feb 15;3(2):177-87. PMID:7735834<ref>PMID:7735834</ref>
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{{ABSTRACT_PUBMED_7735834}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1h4i" style="background-color:#fffaf0;"></div>
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[[1h4i]] is a 4 chain structure of [[Methanol dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4I OCA].
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==See Also==
==See Also==
*[[Methanol dehydrogenase|Methanol dehydrogenase]]
*[[Methanol dehydrogenase|Methanol dehydrogenase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:007735834</ref><ref group="xtra">PMID:011502173</ref><references group="xtra"/>
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__TOC__
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[[Category: Methylobacterium extorquens]]
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</StructureSection>
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[[Category: Anthony, C.]]
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[[Category: Large Structures]]
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[[Category: Blake, C.]]
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[[Category: Methylorubrum extorquens]]
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[[Category: Ghosh, M.]]
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[[Category: Anthony C]]
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[[Category: Goodwin, M G.]]
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[[Category: Blake C]]
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[[Category: Harlos, K.]]
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[[Category: Ghosh M]]
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[[Category: Dehydrogenase]]
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[[Category: Goodwin MG]]
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[[Category: Quinoprotein]]
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[[Category: Harlos K]]

Current revision

Methylobacterium extorquens methanol dehydrogenase

PDB ID 1h4i

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