1hux

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[[Image:1hux.gif|left|200px]]<br /><applet load="1hux" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hux, resolution 3.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE ACIDAMINOCOCCUS FERMENTANS (R)-2-HYDROXYGLUTARYL-COA DEHYDRATASE COMPONENT A'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE ACIDAMINOCOCCUS FERMENTANS (R)-2-HYDROXYGLUTARYL-COA DEHYDRATASE COMPONENT A==
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Acidaminococcus fermentans degrades glutamate via the hydroxyglutarate pathway, which involves the syn-elimination of water from (R)-2-hydroxyglutaryl-CoA in a key reaction of the pathway. This anaerobic process is catalyzed by 2-hydroxyglutaryl-CoA dehydratase, an enzyme with two components (A and D) that reversibly associate during reaction cycles. Component A (CompA), a homodimeric protein of 2x27 kDa, contains a single, bridging [4Fe-4S] cluster and uses the hydrolysis of ATP to deliver an electron to the dehydratase component (CompD), where the electron is used catalytically. The structure of the extremely oxygen-sensitive CompA protein was solved by X-ray crystallography to 3 A resolution. The protein was found to be a member of the actin fold family, revealing a similar architecture and nucleotide-binding site. The key differences between CompA and other members of the actin fold family are: (i) the presence of a cluster binding segment, the "cluster helix"; (ii) the [4Fe-4S] cluster; and (iii) the location of the homodimer interface, which involves the bridging cluster. Possible reaction mechanisms are discussed in light of the close structural similarity to members of the actin-fold family and the functional similarity to the nitrogenase Fe- protein.
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<StructureSection load='1hux' size='340' side='right'caption='[[1hux]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1hux]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidaminococcus_fermentans Acidaminococcus fermentans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HUX FirstGlance]. <br>
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1HUX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acidaminococcus_fermentans Acidaminococcus fermentans] with <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUX OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hux FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hux OCA], [https://pdbe.org/1hux PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hux RCSB], [https://www.ebi.ac.uk/pdbsum/1hux PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hux ProSAT]</span></td></tr>
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Crystal structure of the Acidaminococcus fermentans 2-hydroxyglutaryl-CoA dehydratase component A., Locher KP, Hans M, Yeh AP, Schmid B, Buckel W, Rees DC, J Mol Biol. 2001 Mar 16;307(1):297-308. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11243821 11243821]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HGDC_ACIFV HGDC_ACIFV] Required for the activation of (R)-2-hydroxyglutaryl-CoA dehydratase. This protein is extremely sensitive towards oxygen.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hu/1hux_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hux ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Acidaminococcus fermentans]]
[[Category: Acidaminococcus fermentans]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Buckel, W.]]
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[[Category: Buckel W]]
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[[Category: Hans, M.]]
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[[Category: Hans M]]
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[[Category: Locher, K P.]]
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[[Category: Locher KP]]
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[[Category: Rees, D C.]]
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[[Category: Rees DC]]
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[[Category: Schmid, B.]]
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[[Category: Schmid B]]
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[[Category: Yeh, A P.]]
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[[Category: Yeh AP]]
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[[Category: ADP]]
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[[Category: SF4]]
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[[Category: actin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:05:04 2008''
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Current revision

CRYSTAL STRUCTURE OF THE ACIDAMINOCOCCUS FERMENTANS (R)-2-HYDROXYGLUTARYL-COA DEHYDRATASE COMPONENT A

PDB ID 1hux

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