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1i0j

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[[Image:1i0j.gif|left|200px]]<br /><applet load="1i0j" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i0j, resolution 1.06&Aring;" />
 
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'''1.06 A STRUCTURE OF THE A-DECAMER GCGTATACGC WITH A SINGLE 2'-O-METHYL-3'-METHYLENEPHOSPHONATE (T23) THYMINE IN PLACE OF T6, HIGH CS-SALT'''<br />
 
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==Overview==
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==1.06 A STRUCTURE OF THE A-DECAMER GCGTATACGC WITH A SINGLE 2'-O-METHYL-3'-METHYLENEPHOSPHONATE (T23) THYMINE IN PLACE OF T6, HIGH CS-SALT==
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The observation of light metal ions in nucleic acids crystals is generally a fortuitous event. Sodium ions in particular are notoriously difficult to detect because their X-ray scattering contributions are virtually identical to those of water and Na(+.)O distances are only slightly shorter than strong hydrogen bonds between well-ordered water molecules. We demonstrate here that replacement of Na(+) by K(+), Rb(+) or Cs(+) and precise measurements of anomalous differences in intensities provide a particularly sensitive method for detecting alkali metal ion-binding sites in nucleic acid crystals. Not only can alkali metal ions be readily located in such structures, but the presence of Rb(+) or Cs(+) also allows structure determination by the single wavelength anomalous diffraction technique. Besides allowing identification of high occupancy binding sites, the combination of high resolution and anomalous diffraction data established here can also pinpoint binding sites that feature only partial occupancy. Conversely, high resolution of the data alone does not necessarily allow differentiation between water and partially ordered metal ions, as demonstrated with the crystal structure of a DNA duplex determined to a resolution of 0.6 A.
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<StructureSection load='1i0j' size='340' side='right'caption='[[1i0j]], [[Resolution|resolution]] 1.06&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1i0j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I0J FirstGlance]. <br>
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1I0J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CS:'>CS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0J OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.06&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene>, <scene name='pdbligand=T23:2-O-METHYL-3-METHYL-3-DEOXY-ARABINOFURANOSYL-THYMINE-5-PHOSPHATE'>T23</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i0j OCA], [https://pdbe.org/1i0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i0j RCSB], [https://www.ebi.ac.uk/pdbsum/1i0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i0j ProSAT]</span></td></tr>
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Detection of alkali metal ions in DNA crystals using state-of-the-art X-ray diffraction experiments., Tereshko V, Wilds CJ, Minasov G, Prakash TP, Maier MA, Howard A, Wawrzak Z, Manoharan M, Egli M, Nucleic Acids Res. 2001 Mar 1;29(5):1208-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11222771 11222771]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Egli, M.]]
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</StructureSection>
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[[Category: Howard, A.]]
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[[Category: Large Structures]]
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[[Category: Maier, M A.]]
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[[Category: Egli M]]
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[[Category: Manoharan, M.]]
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[[Category: Howard A]]
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[[Category: Minasov, G.]]
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[[Category: Maier MA]]
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[[Category: Prakash, T P.]]
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[[Category: Manoharan M]]
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[[Category: Tereshko, V.]]
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[[Category: Minasov G]]
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[[Category: Wawrzak, Z.]]
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[[Category: Prakash TP]]
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[[Category: Wilds, C J.]]
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[[Category: Tereshko V]]
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[[Category: CS]]
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[[Category: Wawrzak Z]]
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[[Category: a-form double helix]]
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[[Category: Wilds CJ]]
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[[Category: modified sugar]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:06:41 2008''
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Current revision

1.06 A STRUCTURE OF THE A-DECAMER GCGTATACGC WITH A SINGLE 2'-O-METHYL-3'-METHYLENEPHOSPHONATE (T23) THYMINE IN PLACE OF T6, HIGH CS-SALT

PDB ID 1i0j

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