1i0t

[[Image:1i0t.gif|left|200px]]<br /><applet load="1i0t" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1i0t, resolution 0.60&Aring;" />

'''0.6 A STRUCTURE OF Z-DNA CGCGCG'''<br />

==Overview==

The observation of light metal ions in nucleic acids crystals is generally a fortuitous event. Sodium ions in particular are notoriously difficult to detect because their X-ray scattering contributions are virtually identical to those of water and Na(+.)O distances are only slightly shorter than strong hydrogen bonds between well-ordered water molecules. We demonstrate here that replacement of Na(+) by K(+), Rb(+) or Cs(+) and precise measurements of anomalous differences in intensities provide a particularly sensitive method for detecting alkali metal ion-binding sites in nucleic acid crystals. Not only can alkali metal ions be readily located in such structures, but the presence of Rb(+) or Cs(+) also allows structure determination by the single wavelength anomalous diffraction technique. Besides allowing identification of high occupancy binding sites, the combination of high resolution and anomalous diffraction data established here can also pinpoint binding sites that feature only partial occupancy. Conversely, high resolution of the data alone does not necessarily allow differentiation between water and partially ordered metal ions, as demonstrated with the crystal structure of a DNA duplex determined to a resolution of 0.6 A.

==About this Structure==

1I0T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0T OCA].

 

Detection of alkali metal ions in DNA crystals using state-of-the-art X-ray diffraction experiments., Tereshko V, Wilds CJ, Minasov G, Prakash TP, Maier MA, Howard A, Wawrzak Z, Manoharan M, Egli M, Nucleic Acids Res. 2001 Mar 1;29(5):1208-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11222771 11222771]

[[Category: Protein complex]]

[[Category: Egli, M.]]

[[Category: Howard, A.]]

[[Category: Maier, M A.]]

[[Category: Manoharan, M.]]

[[Category: Minasov, G.]]

[[Category: Prakash, T P.]]

[[Category: Tereshko, V.]]

[[Category: Wawrzak, Z.]]

[[Category: Wilds, C J.]]