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1i1i

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[[Image:1i1i.gif|left|200px]]<br /><applet load="1i1i" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i1i, resolution 2.3&Aring;" />
 
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'''NEUROLYSIN (ENDOPEPTIDASE 24.16) CRYSTAL STRUCTURE'''<br />
 
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==Overview==
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==NEUROLYSIN (ENDOPEPTIDASE 24.16) CRYSTAL STRUCTURE==
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The zinc metallopeptidase neurolysin is shown by x-ray crystallography to have large structural elements erected over the active site region that allow substrate access only through a deep narrow channel. This architecture accounts for specialization of this neuropeptidase to small bioactive peptide substrates without bulky secondary and tertiary structures. In addition, modeling studies indicate that the length of a substrate N-terminal to the site of hydrolysis is restricted to approximately 10 residues by the limited size of the active site cavity. Some structural elements of neurolysin, including a five-stranded beta-sheet and the two active site helices, are conserved with other metallopeptidases. The connecting loop regions of these elements, however, are much extended in neurolysin, and they, together with other open coil elements, line the active site cavity. These potentially flexible elements may account for the ability of the enzyme to cleave a variety of sequences.
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<StructureSection load='1i1i' size='340' side='right'caption='[[1i1i]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1i1i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I1I FirstGlance]. <br>
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1I1I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Neurolysin Neurolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.16 3.4.24.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I1I OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i1i OCA], [https://pdbe.org/1i1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i1i RCSB], [https://www.ebi.ac.uk/pdbsum/1i1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i1i ProSAT]</span></td></tr>
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Structure of neurolysin reveals a deep channel that limits substrate access., Brown CK, Madauss K, Lian W, Beck MR, Tolbert WD, Rodgers DW, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3127-32. Epub 2001 Mar 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11248043 11248043]
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</table>
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[[Category: Neurolysin]]
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== Function ==
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[https://www.uniprot.org/uniprot/NEUL_RAT NEUL_RAT] Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i1/1i1i_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i1i ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Beck MR]]
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[[Category: Beck, M R.]]
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[[Category: Brown CK]]
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[[Category: Brown, C K.]]
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[[Category: Lian W]]
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[[Category: Lian, W.]]
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[[Category: Madauss K]]
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[[Category: Madauss, K.]]
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[[Category: Rodgers DW]]
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[[Category: Rodgers, D W.]]
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[[Category: Tolbert WD]]
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[[Category: Tolbert, W D.]]
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[[Category: ZN]]
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[[Category: endopeptidase]]
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[[Category: neuropeptidase]]
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[[Category: zinc metallopeptidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:06:59 2008''
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Current revision

NEUROLYSIN (ENDOPEPTIDASE 24.16) CRYSTAL STRUCTURE

PDB ID 1i1i

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