2lht

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (05:20, 17 October 2024) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2lht.png|left|200px]]
 
-
{{STRUCTURE_2lht| PDB=2lht | SCENE= }}
+
==Solution structure of Venturia inaequalis cellophane-induced 1 protein (ViCin1) domains 1 and 2==
 +
<StructureSection load='2lht' size='340' side='right'caption='[[2lht]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2lht]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Venturia_inaequalis Venturia inaequalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LHT FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lht OCA], [https://pdbe.org/2lht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lht RCSB], [https://www.ebi.ac.uk/pdbsum/2lht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lht ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/A8W3P3_VENIN A8W3P3_VENIN]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Venturia inaequalis is a hemi-biotrophic fungus that causes scab disease of apple. A recently-identified gene from this fungus, cin1 (cellophane-induced 1), is up-regulated over 1000-fold in planta and considerably on cellophane membranes, and encodes a cysteine-rich secreted protein of 523 residues with eight imperfect tandem repeats of ~60 amino acids. The Cin1 sequence has no homology to known proteins and appears to be genus-specific; however, Cin1 repeats and other repeat domains may be structurally similar. An NMR-derived structure of the first two repeat domains of Cin1 (Cin1-D1D2) and a low-resolution model of the full-length protein (Cin1-FL) using SAXS data were determined. The structure of Cin1-D1D2 reveals that each domain comprises a core helix-loop-helix (HLH) motif as part of a three-helix bundle, and is stabilized by two intra-domain disulfide bonds. Cin1-D1D2 adopts a unique protein fold as DALI and PDBeFOLD analysis identified no structural homology. A (15)N backbone NMR dynamic analysis of Cin1-D1D2 showed that a short stretch of the inter-domain linker has large amplitude motions that give rise to reciprocal domain-domain mobility. This observation was supported by SAXS data modeling, where the scattering length density envelope remains thick at the domain-domain boundary, indicative of inter-domain dynamics. Cin1-FL SAXS data models a loosely-packed arrangement of domains, rather than the canonical parallel packing of adjacent HLH repeats observed in alpha-solenoid repeat proteins. Together, these data suggest that the repeat domains of Cin1 display a "beads-on-a-string" organization with inherent inter-domain flexibility that is likely to facilitate interactions with target ligands.
-
===Solution structure of Venturia inaequalis cellophane-induced 1 protein (ViCin1) domains 1 and 2===
+
Structure, dynamics and domain organization of the repeat protein Cin1 from the apple scab fungus.,Mesarich CH, Schmitz M, Tremouilhac P, McGillivray DJ, Templeton MD, Dingley AJ Biochim Biophys Acta. 2012 Oct;1824(10):1118-28. Epub 2012 Jul 4. PMID:22771296<ref>PMID:22771296</ref>
-
{{ABSTRACT_PUBMED_22771296}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2lht" style="background-color:#fffaf0;"></div>
-
[[2lht]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Venturia_inaequalis Venturia inaequalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LHT OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:022771296</ref><references group="xtra"/>
+
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Venturia inaequalis]]
[[Category: Venturia inaequalis]]
-
[[Category: Dingley, A J.]]
+
[[Category: Dingley AJ]]
-
[[Category: Greenwood, D R.]]
+
[[Category: Greenwood DR]]
-
[[Category: Mcgillivray, D J.]]
+
[[Category: Mcgillivray DJ]]
-
[[Category: Mesarich, C H.]]
+
[[Category: Mesarich CH]]
-
[[Category: Schmitz, M.]]
+
[[Category: Schmitz M]]
-
[[Category: Templeton, M D.]]
+
[[Category: Templeton MD]]
-
[[Category: Tremouilhac, P.]]
+
[[Category: Tremouilhac P]]
-
[[Category: Cell adhesion]]
+
-
[[Category: Secreted repeat domain]]
+

Current revision

Solution structure of Venturia inaequalis cellophane-induced 1 protein (ViCin1) domains 1 and 2

PDB ID 2lht

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lht"
Personal tools