1i97

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[[Image:1i97.gif|left|200px]]<br /><applet load="1i97" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i97, resolution 4.5&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH TETRACYCLINE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH TETRACYCLINE==
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The small ribosomal subunit is responsible for the decoding of genetic information and plays a key role in the initiation of protein synthesis. We analyzed by X-ray crystallography the structures of three different complexes of the small ribosomal subunit of Thermus thermophilus with the A-site inhibitor tetracycline, the universal initiation inhibitor edeine and the C-terminal domain of the translation initiation factor IF3. The crystal structure analysis of the complex with tetracycline revealed the functionally important site responsible for the blockage of the A-site. Five additional tetracycline sites resolve most of the controversial biochemical data on the location of tetracycline. The interaction of edeine with the small subunit indicates its role in inhibiting initiation and shows its involvement with P-site tRNA. The location of the C-terminal domain of IF3, at the solvent side of the platform, sheds light on the formation of the initiation complex, and implies that the anti-association activity of IF3 is due to its influence on the conformational dynamics of the small ribosomal subunit.
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<StructureSection load='1i97' size='340' side='right'caption='[[1i97]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1i97]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I97 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAC:TETRACYCLINE'>TAC</scene>, <scene name='pdbligand=WO2:OCTADECATUNGSTENYL+DIPHOSPHATE'>WO2</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i97 OCA], [https://pdbe.org/1i97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i97 RCSB], [https://www.ebi.ac.uk/pdbsum/1i97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i97 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i9/1i97_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i97 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1I97 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=WO2:'>WO2</scene> and <scene name='pdbligand=TAC:'>TAC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I97 OCA].
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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*[[Ribosome|Ribosome]]
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==Reference==
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3., Pioletti M, Schlunzen F, Harms J, Zarivach R, Gluhmann M, Avila H, Bashan A, Bartels H, Auerbach T, Jacobi C, Hartsch T, Yonath A, Franceschi F, EMBO J. 2001 Apr 17;20(8):1829-39. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11296217 11296217]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Avila, H.]]
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[[Category: Avila H]]
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[[Category: Bartels, H.]]
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[[Category: Bartels H]]
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[[Category: Franceschi, F.]]
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[[Category: Franceschi F]]
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[[Category: Gluehmann, M.]]
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[[Category: Gluehmann M]]
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[[Category: Harms, J.]]
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[[Category: Harms J]]
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[[Category: Hartsch, T.]]
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[[Category: Hartsch T]]
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[[Category: Jacobi, C.]]
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[[Category: Jacobi C]]
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[[Category: Pioletti, M.]]
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[[Category: Pioletti M]]
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[[Category: Schluenzen, F.]]
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[[Category: Schluenzen F]]
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[[Category: Yonath, A.]]
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[[Category: Yonath A]]
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[[Category: Zarivach, R.]]
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[[Category: Zarivach R]]
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[[Category: MG]]
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[[Category: TAC]]
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[[Category: WO2]]
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[[Category: ZN]]
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[[Category: 30s ribosome]]
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[[Category: antibiotics]]
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[[Category: tetracycline]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:19 2008''
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Current revision

CRYSTAL STRUCTURE OF THE 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH TETRACYCLINE

PDB ID 1i97

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