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1nox

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[[Image:1nox.gif|left|200px]]<br />
 
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<applet load="1nox" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nox, resolution 1.59&Aring;" />
 
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'''NADH OXIDASE FROM THERMUS THERMOPHILUS'''<br />
 
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==Overview==
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==NADH OXIDASE FROM THERMUS THERMOPHILUS==
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The crystal structures of the flavin adenine dinucleotide (FAD) and flavin, mononucleotide (FMN) containing isoforms of NADH oxidase from Thermus, thermophilus have been determined by isomorphous and molecular replacement, and refined to 2.3 A and 1.6 A resolution with R-values of 18.5% and 18.6%, respectively. The structure of the homodimeric enzyme consists of a, central 4-stranded antiparallel beta-sheet covered by helices, a more, flexible domain formed by two helices, and a C-terminal excursion, connecting the subunits. The active sites are located in a deep cleft, between the subunits. The binding site of the flavin cofactor lacks the, common nucleotide binding fold and is different from the FMN binding site, found in flavodoxins.
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<StructureSection load='1nox' size='340' side='right'caption='[[1nox]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nox]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NOX FirstGlance]. <br>
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1NOX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]] with FMN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/NADH_dehydrogenase NADH dehydrogenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.3 1.6.99.3]]. Structure known Active Site: COF. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NOX OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nox OCA], [https://pdbe.org/1nox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nox RCSB], [https://www.ebi.ac.uk/pdbsum/1nox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nox ProSAT]</span></td></tr>
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Crystal structure of NADH oxidase from Thermus thermophilus., Hecht HJ, Erdmann H, Park HJ, Sprinzl M, Schmid RD, Nat Struct Biol. 1995 Dec;2(12):1109-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8846223 8846223]
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</table>
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[[Category: NADH dehydrogenase]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/NOX_THET8 NOX_THET8] Can oxidize either NADH or NADPH with a preference for NADH. Can catalyze electron transfer from NADH to various electron acceptors which include, in addition to molecular oxygen, cytochrome c, 2,6 dichlorphenolindophenol, methylene blue, ferricyanide or P-nitroblue tetrazolium.
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[[Category: Thermus thermophilus]]
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== Evolutionary Conservation ==
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[[Category: Erdmann, H.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Hecht, H.J.]]
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Check<jmol>
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[[Category: Park, H.J.]]
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<jmolCheckbox>
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[[Category: Schmid, R.D.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/no/1nox_consurf.spt"</scriptWhenChecked>
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[[Category: Sprinzl, M.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: FMN]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: flavoenzyme ]]
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</jmolCheckbox>
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[[Category: flavoprotein fmn]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nox ConSurf].
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[[Category: oxidoreductase]]
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<div style="clear:both"></div>
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[[Category: thermophile]]
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__TOC__
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</StructureSection>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:19:05 2007''
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Erdmann H]]
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[[Category: Hecht HJ]]
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[[Category: Park HJ]]
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[[Category: Schmid RD]]
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[[Category: Sprinzl M]]

Current revision

NADH OXIDASE FROM THERMUS THERMOPHILUS

PDB ID 1nox

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