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1iez

From Proteopedia

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[[Image:1iez.gif|left|200px]]<br /><applet load="1iez" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1iez" />
 
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'''Solution Structure of 3,4-Dihydroxy-2-Butanone 4-Phosphate Synthase of Riboflavin Biosynthesis'''<br />
 
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==Overview==
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==Solution Structure of 3,4-Dihydroxy-2-Butanone 4-Phosphate Synthase of Riboflavin Biosynthesis==
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Recent developments in NMR have extended the size range of proteins amenable to structural and functional characterization to include many larger proteins involved in important cellular processes. By applying a combination of residue-specific isotope labeling and protein deuteration strategies tailored to yield specific information, we were able to determine the solution structure and study structure-activity relationships of 3,4-dihydroxy-2-butanone-4-phosphate synthase, a 47-kDa enzyme from the Escherichia coli riboflavin biosynthesis pathway and an attractive target for novel antibiotics. Our investigations of the enzyme's ligand binding by NMR and site-directed mutagenesis yields a conclusive picture of the location and identity of residues directly involved in substrate binding and catalysis. Our studies illustrate the power of state-of-the-art NMR techniques for the structural characterization and investigation of ligand binding in protein complexes approaching the 50-kDa range in solution.
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<StructureSection load='1iez' size='340' side='right'caption='[[1iez]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1iez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IEZ FirstGlance]. <br>
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1IEZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IEZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iez OCA], [https://pdbe.org/1iez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iez RCSB], [https://www.ebi.ac.uk/pdbsum/1iez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iez ProSAT]</span></td></tr>
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==Reference==
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</table>
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The NMR structure of the 47-kDa dimeric enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase and ligand binding studies reveal the location of the active site., Kelly MJ, Ball LJ, Krieger C, Yu Y, Fischer M, Schiffmann S, Schmieder P, Kuhne R, Bermel W, Bacher A, Richter G, Oschkinat H, Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13025-30. Epub 2001 Oct 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11687623 11687623]
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== Function ==
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[[Category: Escherichia coli]]
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[https://www.uniprot.org/uniprot/RIBB_ECOLI RIBB_ECOLI] Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.[HAMAP-Rule:MF_00180]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Bacher, A.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Ball, L J.]]
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Check<jmol>
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[[Category: Kelly, M J.S.]]
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<jmolCheckbox>
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[[Category: Kuhne, R.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/1iez_consurf.spt"</scriptWhenChecked>
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[[Category: Oschkinat, H.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: antimicrobial target]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: dihydroxybutanone phosphate synthase]]
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</jmolCheckbox>
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[[Category: riboflavin biosynthesis]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iez ConSurf].
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[[Category: skeletal rearrangement]]
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<div style="clear:both"></div>
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[[Category: structure based design]]
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__TOC__
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</StructureSection>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:12 2008''
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Bacher A]]
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[[Category: Ball LJ]]
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[[Category: Kelly MJS]]
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[[Category: Kuhne R]]
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[[Category: Oschkinat H]]

Current revision

Solution Structure of 3,4-Dihydroxy-2-Butanone 4-Phosphate Synthase of Riboflavin Biosynthesis

PDB ID 1iez

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