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1u0i

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[[Image:1u0i.png|left|200px]]
 
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{{STRUCTURE_1u0i| PDB=1u0i | SCENE= }}
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==IAAL-E3/K3 heterodimer==
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<StructureSection load='1u0i' size='340' side='right'caption='[[1u0i]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1u0i]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0I FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0i OCA], [https://pdbe.org/1u0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0i RCSB], [https://www.ebi.ac.uk/pdbsum/1u0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0i ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The NMR solution structure of a highly stable coiled-coil IAAL-E3/K3 has been solved. The E3/K3 coiled-coil is a 42-residue de novo designed coiled-coil comprising three heptad repeats per subunit, stabilized by hydrophobic contacts within the core and electrostatic interactions at the interface crossing the hydrophobic core which direct heterodimer formation. This E3/K3 domain has previously been shown to have high alpha-helical content as well as possessing a low dissociation constant (70 nM). The E3/K3 structure is completely alpha-helical and is an archetypical coiled-coil in solution, as determined using a combination of (1)H-NOE and homology based structural restraints. This structure provides a structural framework for visualizing the important interactions for stability and specificity, which are key to protein engineering applications such as affinity purification and de novo design.
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===IAAL-E3/K3 heterodimer===
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NMR solution structure of a highly stable de novo heterodimeric coiled-coil.,Lindhout DA, Litowski JR, Mercier P, Hodges RS, Sykes BD Biopolymers. 2004 Dec 5;75(5):367-75. PMID:15457434<ref>PMID:15457434</ref>
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{{ABSTRACT_PUBMED_15457434}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1u0i" style="background-color:#fffaf0;"></div>
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[[1u0i]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0I OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:015457434</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Hodges, R S.]]
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[[Category: Large Structures]]
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[[Category: Lindhout, D A.]]
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[[Category: Hodges RS]]
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[[Category: Litowski, J R.]]
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[[Category: Lindhout DA]]
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[[Category: Mercier, P.]]
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[[Category: Litowski JR]]
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[[Category: Sykes, B D.]]
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[[Category: Mercier P]]
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[[Category: Coiled-coil]]
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[[Category: Sykes BD]]
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[[Category: De novo protein]]
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Current revision

IAAL-E3/K3 heterodimer

PDB ID 1u0i

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