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1ifa

From Proteopedia

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[[Image:1ifa.jpg|left|200px]]<br /><applet load="1ifa" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ifa, resolution 2.6&Aring;" />
 
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'''THREE-DIMENSIONAL CRYSTAL STRUCTURE OF RECOMBINANT MURINE INTERFERON-BETA'''<br />
 
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==Overview==
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==THREE-DIMENSIONAL CRYSTAL STRUCTURE OF RECOMBINANT MURINE INTERFERON-BETA==
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The crystal structure of recombinant murine interferon-beta (IFN-beta) has been solved by the multiple isomorphous replacement method and refined to an R-factor of 20.5% against 2.6 A X-ray diffraction data. The structure shows a variant of the alpha-helix bundle with a new chain-folding topology, which seems to represent a basic structural framework of all the IFN-alpha and IFN-beta molecules belonging to the type I family. Functionally important segments of the polypeptide chain, as implied through numerous gene manipulation studies carried out so far, are spatially clustered indicating the binding site(s) to the receptor(s). Comparison of the present structure with those of other alpha-helical cytokine proteins, including porcine growth hormone, interleukin 2 and interferon gamma, indicated either a topological similarity in chain folding or a similar spatial arrangement of the alpha-helices.
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<StructureSection load='1ifa' size='340' side='right'caption='[[1ifa]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ifa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IFA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ifa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ifa OCA], [https://pdbe.org/1ifa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ifa RCSB], [https://www.ebi.ac.uk/pdbsum/1ifa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ifa ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IFNB_MOUSE IFNB_MOUSE] Has antiviral, antibacterial and anticancer activities.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/if/1ifa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ifa ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1IFA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ASN:'>ASN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IFA OCA].
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*[[Interferon 3D structures|Interferon 3D structures]]
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*[[Multiple sclerosis|Multiple sclerosis]]
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==Reference==
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__TOC__
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Three-dimensional crystal structure of recombinant murine interferon-beta., Senda T, Shimazu T, Matsuda S, Kawano G, Shimizu H, Nakamura KT, Mitsui Y, EMBO J. 1992 Sep;11(9):3193-201. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1505514 1505514]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Kawano G]]
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[[Category: Kawano, G.]]
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[[Category: Matsuda S]]
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[[Category: Matsuda, S.]]
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[[Category: Mitsui Y]]
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[[Category: Mitsui, Y.]]
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[[Category: Nakamura KT]]
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[[Category: Nakamura, K T.]]
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[[Category: Senda T]]
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[[Category: Senda, T.]]
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[[Category: Shimizu H]]
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[[Category: Shimizu, H.]]
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[[Category: ASN]]
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[[Category: glycoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:20 2008''
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Current revision

THREE-DIMENSIONAL CRYSTAL STRUCTURE OF RECOMBINANT MURINE INTERFERON-BETA

PDB ID 1ifa

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