1k64

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[[Image:1k64.png|left|200px]]
 
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{{STRUCTURE_1k64| PDB=1k64 | SCENE= }}
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==NMR Structue of alpha-conotoxin EI==
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<StructureSection load='1k64' size='340' side='right'caption='[[1k64]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k64]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_ermineus Conus ermineus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K64 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K64 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k64 OCA], [https://pdbe.org/1k64 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k64 RCSB], [https://www.ebi.ac.uk/pdbsum/1k64 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k64 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CA1A_CONER CA1A_CONER] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. Probable competitive antagonist of fish muscle acetylcholine receptor (PubMed:32245200). Inhibits postsynaptic nicotinic acetylcholine receptors (nAChRs) from zebrafish (IC(50)=58-100 nM) (PubMed:32245200). Blocks mammalian nAChR composed of alpha-1/gamma and alpha-1/delta subunits. Blocks central nervous system nAChR composed of alpha-4-beta-2/CHRNA4-CHRNB2 subunits and peripheral nervous system nAChR composed of alpha-3-beta-4/CHRNA3-CHRNB4 subunits. Low toxin concentrations potentiate currents in muscle nAChR composed of alpha-1-beta-1-gamma-delta (CHRNA1-CHRNB1-CHRNG-CHRND) subunits and central nervous system nAChR composed of alpha-4-beta-2/CHRNA4-CHRNB2 subunits, but not peripheral nervous system nAChR composed of alpha-3-beta-4 subunits. Also exhibits inhibition of D.melanogaster alpha-7/CHRNA7 nAChRs (PubMed:25466886). Has possibly a distinct nAChR binding mode from other alpha-conotoxins, due to a different three residue motif (lacks the Ser-Xaa-Pro motif) (By similarity).[UniProtKB:Q2I2R8]<ref>PMID:17635581</ref> <ref>PMID:25466886</ref> <ref>PMID:32245200</ref> <ref>PMID:7578057</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A high resolution structure of alpha-conotoxin EI has been determined by (1)H NMR spectroscopy and molecular modeling. alpha-Conotoxin EI has the same disulfide framework as alpha 4/7 conotoxins targeting neuronal nicotinic acetylcholine receptors but antagonizes the neuromuscular receptor as do the alpha 3/5 and alpha A conotoxins. The unique binding preference of alpha-conotoxin EI to the alpha(1)/delta subunit interface of Torpedo neuromuscular receptor makes it a valuable structural template for superposition of various alpha-conotoxins possessing distinct receptor subtype specificities. Structural comparison of alpha-conotoxin EI with the gamma-subunit favoring alpha-conotoxin GI suggests that the Torpedo delta-subunit preference of the former originates from its second loop. Superposition of three-dimensional structures of seven alpha-conotoxins reveals that the estimated size of the toxin-binding pocket in nicotinic acetylcholine receptor is approximately 20 A (height) x 20 A (width) x 15 A (thickness).
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===NMR Structue of alpha-conotoxin EI===
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Solution conformation of alpha-conotoxin EI, a neuromuscular toxin specific for the alpha 1/delta subunit interface of torpedo nicotinic acetylcholine receptor.,Park KH, Suk JE, Jacobsen R, Gray WR, McIntosh JM, Han KH J Biol Chem. 2001 Dec 28;276(52):49028-33. Epub 2001 Oct 18. PMID:11641403<ref>PMID:11641403</ref>
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{{ABSTRACT_PUBMED_11641403}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1k64" style="background-color:#fffaf0;"></div>
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[[1k64]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K64 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:011641403</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Gray, W R.]]
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[[Category: Conus ermineus]]
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[[Category: Han, K H.]]
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[[Category: Large Structures]]
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[[Category: Jacobsen, R.]]
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[[Category: Gray WR]]
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[[Category: McIntosh, J M.]]
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[[Category: Han KH]]
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[[Category: Park, K H.]]
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[[Category: Jacobsen R]]
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[[Category: Suk, J E.]]
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[[Category: McIntosh JM]]
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[[Category: Omega-shaped containing a-helix]]
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[[Category: Park KH]]
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[[Category: Toxin]]
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[[Category: Suk JE]]

Current revision

NMR Structue of alpha-conotoxin EI

PDB ID 1k64

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