1ija

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[[Image:1ija.jpg|left|200px]]<br /><applet load="1ija" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ija" />
 
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'''Structure of Sortase'''<br />
 
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==Overview==
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==Structure of Sortase==
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Surface proteins of Gram-positive bacteria play important roles during the pathogenesis of human infections and require sortase for anchoring to the cell-wall envelope. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine (T) and the amino group of cell-wall crossbridges. The NMR structure of sortase reveals a unique beta-barrel structure, in which the active-site sulfhydryl of cysteine-184 is poised for ionization by histidine-120, presumably enabling the resultant thiolate to attack the LPXTG peptide. Calcium binding near the active site stimulates catalysis, possibly by altering the conformation of a surface loop that recognizes newly translocated polypeptides. The structure suggests a mechanistic relationship to the papain/cathepsin proteases and should facilitate the design of new antiinfective agents.
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<StructureSection load='1ija' size='340' side='right'caption='[[1ija]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ija]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IJA FirstGlance]. <br>
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1IJA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ija FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ija OCA], [https://pdbe.org/1ija PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ija RCSB], [https://www.ebi.ac.uk/pdbsum/1ija PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ija ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus., Ilangovan U, Ton-That H, Iwahara J, Schneewind O, Clubb RT, Proc Natl Acad Sci U S A. 2001 May 22;98(11):6056-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11371637 11371637]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q9S446_STAAU Q9S446_STAAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ij/1ija_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ija ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Clubb, R T.]]
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[[Category: Clubb RT]]
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[[Category: Ilangovan, U.]]
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[[Category: Ilangovan U]]
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[[Category: Iwahara, J.]]
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[[Category: Iwahara J]]
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[[Category: Schneewind, O.]]
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[[Category: Schneewind O]]
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[[Category: Ton-That, H.]]
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[[Category: Ton-That H]]
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[[Category: eight stranded beta barrel]]
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[[Category: transpeptidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:12:34 2008''
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Current revision

Structure of Sortase

PDB ID 1ija

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