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1o8z

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[[Image:1o8z.png|left|200px]]
 
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{{STRUCTURE_1o8z| PDB=1o8z | SCENE= }}
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==Solution structure of SFTI-1(6,5), an acyclic permutant of the proteinase inhibitor SFTI-1, cis-trans-trans conformer (ct-A)==
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<StructureSection load='1o8z' size='340' side='right'caption='[[1o8z]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1o8z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helianthus_annuus Helianthus annuus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O8Z FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o8z OCA], [https://pdbe.org/1o8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o8z RCSB], [https://www.ebi.ac.uk/pdbsum/1o8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o8z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SFTI1_HELAN SFTI1_HELAN] Inhibits trypsin, cathepsin G, elastase, chymotrypsin and thrombin. Does not inhibit factor Xa.<ref>PMID:10390350</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The most potent known naturally occurring Bowman-Birk inhibitor, sunflower trypsin inhibitor-1 (SFTI-1), is a bicyclic 14-amino acid peptide from sunflower seeds comprising one disulfide bond and a cyclic backbone. At present, little is known about the cyclization mechanism of SFTI-1. We show here that an acyclic permutant of SFTI-1 open at its scissile bond, SFTI-1[6,5], also functions as an inhibitor of trypsin and that it can be enzymatically backbone-cyclized by incubation with bovine beta-trypsin. The resulting ratio of cyclic SFTI-1 to SFTI-1[6,5] is approximately 9:1 regardless of whether trypsin is incubated with SFTI-1[6,5] or SFTI-1. Enzymatic resynthesis of the scissile bond to form cyclic SFTI-1 is a novel mechanism of cyclization of SFTI-1[6,5]. Such a reaction could potentially occur on a trypsin affinity column as used in the original isolation procedure of SFTI-1. We therefore extracted SFTI-1 from sunflower seeds without a trypsin purification step and confirmed that the backbone of SFTI-1 is indeed naturally cyclic. Structural studies on SFTI-1[6,5] revealed high heterogeneity, and multiple species of SFTI-1[6,5] were identified. The main species closely resembles the structure of cyclic SFTI-1 with the broken binding loop able to rotate between a cis/trans geometry of the I7-P8 bond with the cis conformer being similar to the canonical binding loop conformation. The non-reactive loop adopts a beta-hairpin structure as in cyclic wild-type SFTI-1. Another species exhibits an iso-aspartate residue at position 14 and provides implications for possible in vivo cyclization mechanisms.
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===SOLUTION STRUCTURE OF SFTI-1(6,5), AN ACYCLIC PERMUTANT OF THE PROTEINASE INHIBITOR SFTI-1, CIS-TRANS-TRANS CONFORMER (CT-A)===
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Enzymatic cyclization of a potent bowman-birk protease inhibitor, sunflower trypsin inhibitor-1, and solution structure of an acyclic precursor peptide.,Marx UC, Korsinczky ML, Schirra HJ, Jones A, Condie B, Otvos L Jr, Craik DJ J Biol Chem. 2003 Jun 13;278(24):21782-9. Epub 2003 Mar 5. PMID:12621047<ref>PMID:12621047</ref>
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{{ABSTRACT_PUBMED_12621047}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1o8z" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[1o8z]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O8Z OCA].
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*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:012621047</ref><references group="xtra"/>
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__TOC__
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[[Category: Craik, D J.]]
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</StructureSection>
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[[Category: Marx, U C.]]
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[[Category: Helianthus annuus]]
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[[Category: Acyclic permutant]]
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[[Category: Large Structures]]
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[[Category: Bowman-birk inhibitor]]
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[[Category: Craik DJ]]
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[[Category: Protease inhibitor]]
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[[Category: Marx UC]]
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[[Category: Sfti-1]]
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Current revision

Solution structure of SFTI-1(6,5), an acyclic permutant of the proteinase inhibitor SFTI-1, cis-trans-trans conformer (ct-A)

PDB ID 1o8z

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