1itu

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[[Image:1itu.gif|left|200px]]<br /><applet load="1itu" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1itu, resolution 2.00&Aring;" />
 
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'''HUMAN RENAL DIPEPTIDASE COMPLEXED WITH CILASTATIN'''<br />
 
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==Overview==
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==HUMAN RENAL DIPEPTIDASE COMPLEXED WITH CILASTATIN==
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Human renal dipeptidase is a membrane-bound glycoprotein hydrolyzing dipeptides and is involved in hydrolytic metabolism of penem and carbapenem beta-lactam antibiotics. The crystal structures of the saccharide-trimmed enzyme are determined as unliganded and inhibitor-liganded forms. They are informative for designing new antibiotics that are not hydrolyzed by this enzyme. The active site in each of the (alpha/beta)(8) barrel subunits of the homodimeric molecule is composed of binuclear zinc ions bridged by the Glu125 side-chain located at the bottom of the barrel, and it faces toward the microvillar membrane of a kidney tubule. A dipeptidyl moiety of the therapeutically used cilastatin inhibitor is fully accommodated in the active-site pocket, which is small enough for precise recognition of dipeptide substrates. The barrel and active-site architectures utilizing catalytic metal ions exhibit unexpected similarities to those of the murine adenosine deaminase and the catalytic domain of the bacterial urease.
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<StructureSection load='1itu' size='340' side='right'caption='[[1itu]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1itu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ITU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ITU FirstGlance]. <br>
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Known disease associated with this structure: Dystonia-12 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=182350 182350]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIL:CILASTATIN'>CIL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1itu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1itu OCA], [https://pdbe.org/1itu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1itu RCSB], [https://www.ebi.ac.uk/pdbsum/1itu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1itu ProSAT]</span></td></tr>
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1ITU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CIL:'>CIL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Membrane_dipeptidase Membrane dipeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.13.19 3.4.13.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ITU OCA].
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</table>
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== Function ==
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==Reference==
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[https://www.uniprot.org/uniprot/DPEP1_HUMAN DPEP1_HUMAN] Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.
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Crystal structure of human renal dipeptidase involved in beta-lactam hydrolysis., Nitanai Y, Satow Y, Adachi H, Tsujimoto M, J Mol Biol. 2002 Aug 9;321(2):177-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12144777 12144777]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/1itu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1itu ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Membrane dipeptidase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Adachi H]]
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[[Category: Adachi, H.]]
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[[Category: Nitanai Y]]
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[[Category: Nitanai, Y.]]
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[[Category: Satow Y]]
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[[Category: Satow, Y.]]
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[[Category: Tsujimoto M]]
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[[Category: Tsujimoto, M.]]
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[[Category: CIL]]
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[[Category: NAG]]
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[[Category: ZN]]
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[[Category: cilastatin]]
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[[Category: complex (hydrolase/inhibitor)]]
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[[Category: dipeptidase]]
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[[Category: glycoprotein]]
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[[Category: membrane-bound]]
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[[Category: zinc protease beta-lactamase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:15:35 2008''
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Current revision

HUMAN RENAL DIPEPTIDASE COMPLEXED WITH CILASTATIN

PDB ID 1itu

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