4ag7
From Proteopedia
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| - | [[Image:4ag7.png|left|200px]] | ||
| - | + | ==C. elegans glucosamine-6-phosphate N-acetyltransferase (GNA1): coenzyme A adduct== | |
| + | <StructureSection load='4ag7' size='340' side='right'caption='[[4ag7]], [[Resolution|resolution]] 1.55Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4ag7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AG7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4ag9|4ag9]]</div></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucosamine-phosphate_N-acetyltransferase Glucosamine-phosphate N-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.4 2.3.1.4] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ag7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ag7 OCA], [https://pdbe.org/4ag7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ag7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ag7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ag7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glucosamine-6-phosphate N-acetyltransferase 1 (GNA1) produces GlcNAc-6-phosphate from GlcN-6-phosphate and acetyl coenzyme A. Early mercury-labelling experiments implicated a conserved cysteine in the reaction mechanism, whereas recent structural data appear to support a mechanism in which this cysteine plays no role. Here, two crystal structures of Caenorhabditis elegans GNA1 are reported, revealing an unusual covalent complex between this cysteine and the coenzyme A product. Mass-spectrometric and reduction studies showed that this inactive covalent complex can be reactivated through reduction, yet mutagenesis of the cysteine supports a previously reported bi-bi mechanism. The data unify the apparently contradictory earlier reports on the role of a cysteine in the GNA1 active site. | ||
| - | + | Structural and biochemical characterization of a trapped coenzyme A adduct of Caenorhabditis elegans glucosamine-6-phosphate N-acetyltransferase 1.,Dorfmueller HC, Fang W, Rao FV, Blair DE, Attrill H, van Aalten DM Acta Crystallogr D Biol Crystallogr. 2012 Aug;68(Pt 8):1019-29. Epub 2012 Jul 17. PMID:22868768<ref>PMID:22868768</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 4ag7" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | < | + | </StructureSection> |
| - | [[Category: | + | [[Category: Caeel]] |
[[Category: Glucosamine-phosphate N-acetyltransferase]] | [[Category: Glucosamine-phosphate N-acetyltransferase]] | ||
| - | [[Category: Aalten, D M.F | + | [[Category: Large Structures]] |
| - | [[Category: Attrill, H | + | [[Category: Aalten, D M.F van]] |
| - | [[Category: Blair, D E | + | [[Category: Attrill, H]] |
| - | [[Category: Dorfmueller, H C | + | [[Category: Blair, D E]] |
| - | [[Category: Fang, W | + | [[Category: Dorfmueller, H C]] |
| - | [[Category: Rao, F V | + | [[Category: Fang, W]] |
| - | [[Category: Shepherd, S M | + | [[Category: Rao, F V]] |
| + | [[Category: Shepherd, S M]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Current revision
C. elegans glucosamine-6-phosphate N-acetyltransferase (GNA1): coenzyme A adduct
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