4ag9

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[[Image:4ag9.png|left|200px]]
 
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{{STRUCTURE_4ag9| PDB=4ag9 | SCENE= }}
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==C. elegans glucosamine-6-phosphate N-acetyltransferase (GNA1): ternary complex with coenzyme A and GlcNAc==
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<StructureSection load='4ag9' size='340' side='right'caption='[[4ag9]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ag9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AG9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=16G:N-ACETYL-D-GLUCOSAMINE-6-PHOSPHATE'>16G</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ag9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ag9 OCA], [https://pdbe.org/4ag9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ag9 RCSB], [https://www.ebi.ac.uk/pdbsum/4ag9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ag9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GNA1_CAEEL GNA1_CAEEL]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glucosamine-6-phosphate N-acetyltransferase 1 (GNA1) produces GlcNAc-6-phosphate from GlcN-6-phosphate and acetyl coenzyme A. Early mercury-labelling experiments implicated a conserved cysteine in the reaction mechanism, whereas recent structural data appear to support a mechanism in which this cysteine plays no role. Here, two crystal structures of Caenorhabditis elegans GNA1 are reported, revealing an unusual covalent complex between this cysteine and the coenzyme A product. Mass-spectrometric and reduction studies showed that this inactive covalent complex can be reactivated through reduction, yet mutagenesis of the cysteine supports a previously reported bi-bi mechanism. The data unify the apparently contradictory earlier reports on the role of a cysteine in the GNA1 active site.
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===C. elegans glucosamine-6-phosphate N-acetyltransferase (GNA1): ternary complex with coenzyme A and GlcNAc===
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Structural and biochemical characterization of a trapped coenzyme A adduct of Caenorhabditis elegans glucosamine-6-phosphate N-acetyltransferase 1.,Dorfmueller HC, Fang W, Rao FV, Blair DE, Attrill H, van Aalten DM Acta Crystallogr D Biol Crystallogr. 2012 Aug;68(Pt 8):1019-29. Epub 2012 Jul 17. PMID:22868768<ref>PMID:22868768</ref>
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{{ABSTRACT_PUBMED_22868768}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 4ag9" style="background-color:#fffaf0;"></div>
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[[4ag9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AG9 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:022868768</ref><references group="xtra"/>
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</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Caenorhabditis elegans]]
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[[Category: Glucosamine-phosphate N-acetyltransferase]]
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[[Category: Large Structures]]
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[[Category: Aalten, D M.F Van.]]
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[[Category: Attrill H]]
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[[Category: Attrill, H.]]
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[[Category: Blair DE]]
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[[Category: Blair, D E.]]
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[[Category: Dorfmueller HC]]
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[[Category: Dorfmueller, H C.]]
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[[Category: Fang W]]
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[[Category: Fang, W.]]
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[[Category: Rao FV]]
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[[Category: Rao, F V.]]
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[[Category: Shepherd SM]]
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[[Category: Shepherd, S M.]]
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[[Category: Van Aalten DMF]]
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[[Category: Transferase]]
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Current revision

C. elegans glucosamine-6-phosphate N-acetyltransferase (GNA1): ternary complex with coenzyme A and GlcNAc

PDB ID 4ag9

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