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1j6z

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[[Image:1j6z.gif|left|200px]]<br /><applet load="1j6z" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1j6z, resolution 1.54&Aring;" />
 
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'''UNCOMPLEXED ACTIN'''<br />
 
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==Overview==
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==UNCOMPLEXED ACTIN==
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<StructureSection load='1j6z' size='340' side='right'caption='[[1j6z]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1j6z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J6Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J6Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=RHO:TETRAMETHYLRHODAMINE-5-MALEIMIDE'>RHO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j6z OCA], [https://pdbe.org/1j6z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j6z RCSB], [https://www.ebi.ac.uk/pdbsum/1j6z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j6z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The dynamics and polarity of actin filaments are controlled by a conformational change coupled to the hydrolysis of adenosine 5'-triphosphate (ATP) by a mechanism that remains to be elucidated. Actin modified to block polymerization was crystallized in the adenosine 5'-diphosphate (ADP) state, and the structure was solved to 1.54 angstrom resolution. Compared with previous ATP-actin structures from complexes with deoxyribonuclease I, profilin, and gelsolin, monomeric ADP-actin is characterized by a marked conformational change in subdomain 2. The successful crystallization of monomeric actin opens the way to future structure determinations of actin complexes with actin-binding proteins such as myosin.
The dynamics and polarity of actin filaments are controlled by a conformational change coupled to the hydrolysis of adenosine 5'-triphosphate (ATP) by a mechanism that remains to be elucidated. Actin modified to block polymerization was crystallized in the adenosine 5'-diphosphate (ADP) state, and the structure was solved to 1.54 angstrom resolution. Compared with previous ATP-actin structures from complexes with deoxyribonuclease I, profilin, and gelsolin, monomeric ADP-actin is characterized by a marked conformational change in subdomain 2. The successful crystallization of monomeric actin opens the way to future structure determinations of actin complexes with actin-binding proteins such as myosin.
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==About this Structure==
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The crystal structure of uncomplexed actin in the ADP state.,Otterbein LR, Graceffa P, Dominguez R Science. 2001 Jul 27;293(5530):708-11. PMID:11474115<ref>PMID:11474115</ref>
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1J6Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=RHO:'>RHO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J6Z OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The crystal structure of uncomplexed actin in the ADP state., Otterbein LR, Graceffa P, Dominguez R, Science. 2001 Jul 27;293(5530):708-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11474115 11474115]
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</div>
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[[Category: Oryctolagus cuniculus]]
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<div class="pdbe-citations 1j6z" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Dominguez, R.]]
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[[Category: Graceffa, P.]]
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[[Category: Otterbein, L R.]]
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[[Category: ADP]]
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[[Category: CA]]
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[[Category: RHO]]
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[[Category: actin]]
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[[Category: adp-state]]
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[[Category: tetramethylrhodamine-5-maleimide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:19:21 2008''
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==See Also==
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*[[Actin 3D structures|Actin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Oryctolagus cuniculus]]
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[[Category: Dominguez R]]
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[[Category: Graceffa P]]
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[[Category: Otterbein LR]]

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UNCOMPLEXED ACTIN

PDB ID 1j6z

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