2rsn

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[[Image:2rsn.jpg|left|200px]]
 
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{{STRUCTURE_2rsn| PDB=2rsn | SCENE= }}
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==Solution structure of the chromodomain of Chp1 in complex with H3K9me3 peptide==
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<StructureSection load='2rsn' size='340' side='right'caption='[[2rsn]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2rsn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] and [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RSN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rsn OCA], [https://pdbe.org/2rsn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rsn RCSB], [https://www.ebi.ac.uk/pdbsum/2rsn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rsn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHP1_SCHPO CHP1_SCHPO] Component of the kinetochore which plays a role in stabilizing microtubules and so allowing accurate chromosome segregation. Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. A member of the RNA-induced transcriptional silencing (RITS) complex which is involved in the biosynthesis of dsRNA from primer siRNAs provided by the RNA-directed RNA polymerase (RDRC) complex.<ref>PMID:9722643</ref> <ref>PMID:10835380</ref> <ref>PMID:15607976</ref> <ref>PMID:14704433</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Centromeric heterochromatin assembly in fission yeast requires the RNAi pathway. Chp1, a chromodomain (CD) protein, forms the Ago1-containing RNA-induced transcriptional silencing (RITS) complex and recruits siRNA-bound RITS to methylated histone H3 lysine 9 (H3K9me) via its CD. Here, we show that the CD of Chp1 (Chp1-CD) possesses unique nucleic acid-binding activities that are essential for heterochromatic gene silencing. Detailed electrophoretic-mobility shift analyses demonstrated that Chp1 binds to RNA via the CD in addition to its central RNA-recognition motif. Interestingly, robust RNA- and DNA-binding activity of Chp1-CD was strongly enhanced when it was bound to H3K9me, which was revealed to involve a positively charged domain within the Chp1-CD by structural analyses. These results demonstrate a role for the CD that provides a link between RNA, DNA, and methylated histone tails to ensure heterochromatic gene silencing.
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===Solution structure of the chromodomain of Chp1 in complex with H3K9me3 peptide===
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Intrinsic nucleic Acid-binding activity of chp1 chromodomain is required for heterochromatic gene silencing.,Ishida M, Shimojo H, Hayashi A, Kawaguchi R, Ohtani Y, Uegaki K, Nishimura Y, Nakayama J Mol Cell. 2012 Jul 27;47(2):228-41. Epub 2012 Jun 21. PMID:22727667<ref>PMID:22727667</ref>
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{{ABSTRACT_PUBMED_22727667}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2rsn" style="background-color:#fffaf0;"></div>
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[[2rsn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RSN OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:022727667</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Nishimura, Y.]]
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[[Category: Schizosaccharomyces pombe 972h-]]
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[[Category: Shimojo, H.]]
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[[Category: Nishimura Y]]
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[[Category: Chromodomain]]
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[[Category: Shimojo H]]
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[[Category: Chromosomal protein]]
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[[Category: Methylation]]
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[[Category: Nuclear protein]]
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[[Category: Protein-peptide complex]]
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[[Category: Rna-mediated gene silencing]]
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Current revision

Solution structure of the chromodomain of Chp1 in complex with H3K9me3 peptide

PDB ID 2rsn

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