This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1jic

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:31, 1 May 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1jic.gif|left|200px]]<br /><applet load="1jic" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1jic" />
 
-
'''SOLUTION NMR STRUCTURE OF RECOMBINANT SSO7D WITH RNASE ACTIVITY, MINIMIZED AVERAGE STRUCTURE'''<br />
 
-
==Overview==
+
==SOLUTION NMR STRUCTURE OF RECOMBINANT SSO7D WITH RNASE ACTIVITY, MINIMIZED AVERAGE STRUCTURE==
-
Sso7d is a basic 7-kDa DNA-binding protein from Sulfolobus solfataricus, also endowed with ribonuclease activity. The protein consists of a double-stranded antiparallel beta-sheet, onto which an orthogonal triple-stranded antiparallel beta-sheet is packed, and of a small helical stretch at the C-terminus. Furthermore, the two beta-sheets enclose an aromatic cluster displaying a fishbone geometry. We previously cloned the Sso7d-encoding gene, expressed it in Escherichia coli, and produced several single-point mutants, either of residues located in the hydrophobic core or of Trp23, which is exposed to the solvent and plays a major role in DNA binding. The mutation F31A was dramatically destabilizing, with a loss in thermo- and piezostabilities by at least 27 K and 10 kbar, respectively. Here, we report the solution structure of the F31A mutant, which was determined by NMR spectroscopy using 744 distance constraints obtained from analysis of multidimensional spectra in conjunction with simulated annealing protocols. The most remarkable finding is the change in orientation of the Trp23 side chain, which in the wild type is completely exposed to the solvent, whereas in the mutant is largely buried in the aromatic cluster. This prevents the formation of a cavity in the hydrophobic core of the mutant, which would arise in the absence of structural rearrangements. We found additional changes produced by the mutation, notably a strong distortion in the beta-sheets with loss in several hydrogen bonds, increased flexibility of some stretches of the backbone, and some local strains. On one hand, these features may justify the dramatic destabilization provoked by the mutation; on the other hand, they highlight the crucial role of the hydrophobic core in protein stability. To the best of our knowledge, no similar rearrangement has been so far described as a result of a single-point mutation.
+
<StructureSection load='1jic' size='340' side='right'caption='[[1jic]]' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1jic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JIC FirstGlance]. <br>
-
1JIC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JIC OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jic OCA], [https://pdbe.org/1jic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jic RCSB], [https://www.ebi.ac.uk/pdbsum/1jic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jic ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
A single-point mutation in the extreme heat- and pressure-resistant sso7d protein from sulfolobus solfataricus leads to a major rearrangement of the hydrophobic core., Consonni R, Santomo L, Fusi P, Tortora P, Zetta L, Biochemistry. 1999 Sep 28;38(39):12709-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10504241 10504241]
+
== Function ==
-
[[Category: Single protein]]
+
[https://www.uniprot.org/uniprot/DN7A_SACS2 DN7A_SACS2]
-
[[Category: Sulfolobus solfataricus]]
+
__TOC__
-
[[Category: Consonni, R.]]
+
</StructureSection>
-
[[Category: Santomo, L.]]
+
[[Category: Large Structures]]
-
[[Category: Zetta, L.]]
+
[[Category: Saccharolobus solfataricus]]
-
[[Category: 3d-structure]]
+
[[Category: Consonni R]]
-
[[Category: dna-binding]]
+
[[Category: Santomo L]]
-
[[Category: nmr]]
+
[[Category: Zetta L]]
-
[[Category: rnase and dna-binding protein]]
+
-
[[Category: sulfolobus solfataricus]]
+
-
[[Category: thermostable ribonuclease]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:57 2008''
+

Current revision

SOLUTION NMR STRUCTURE OF RECOMBINANT SSO7D WITH RNASE ACTIVITY, MINIMIZED AVERAGE STRUCTURE

PDB ID 1jic

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jic"
Personal tools