1jj8

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[[Image:1jj8.gif|left|200px]]<br /><applet load="1jj8" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jj8, resolution 2.75&Aring;" />
 
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'''Testing the Water-Mediated HIN Recombinase DNA Recognition by Systematic Mutations'''<br />
 
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==Overview==
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==Testing the Water-Mediated HIN Recombinase DNA Recognition by Systematic Mutations==
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The Hin recombinase specifically recognizes its DNA-binding site by means of both major and minor groove interactions. A previous X-ray structure, together with new structures of the Hin DNA-binding domain bound to a recombination half-site that were solved as part of the present study, have revealed that two ordered water molecules are present within the major groove interface. In this report, we test the importance of these waters directly by X-ray crystal structure analysis of complexes with four mutant DNA sequences. These structures, combined with their Hin-binding properties, provide strong support for the critical importance of one of the intermediate waters. A lesser but demonstrable role is ascribed to the second water molecule. The mutant structures also illustrate the prominent roles of thymine methyls both in stabilizing intermediate waters and in interfering with water or amino acid side chain interactions with DNA.
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<StructureSection load='1jj8' size='340' side='right'caption='[[1jj8]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jj8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJ8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5IU:5-IODO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>5IU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jj8 OCA], [https://pdbe.org/1jj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1jj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jj8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HIN_SALTY HIN_SALTY] A DNA fragment of approximately 900 base pairs, adjacent to the fljB (H2) gene, which specifies the synthesis of phase-2 flagellin, can exist in either orientation with respect to fljB. The orientation of the inversion region controls expression of fljB. The hin gene occupies about two-thirds of the inversion region; it is required for the inversion of the fljB controlling region.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/1jj8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jj8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JJ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJ8 OCA].
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Testing water-mediated DNA recognition by the Hin recombinase., Chiu TK, Sohn C, Dickerson RE, Johnson RC, EMBO J. 2002 Feb 15;21(4):801-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11847127 11847127]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
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[[Category: Chiu, T K.]]
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[[Category: Chiu TK]]
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[[Category: Dickerson, R E.]]
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[[Category: Dickerson RE]]
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[[Category: Johnson, R C.]]
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[[Category: Johnson RC]]
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[[Category: Sohn, C.]]
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[[Category: Sohn C]]
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[[Category: hin recombinase]]
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[[Category: i4 form 2]]
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[[Category: protein-dna complex]]
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[[Category: water-mediated recognition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:20 2008''
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Current revision

Testing the Water-Mediated HIN Recombinase DNA Recognition by Systematic Mutations

PDB ID 1jj8

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