1jjh

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[[Image:1jjh.gif|left|200px]]<br /><applet load="1jjh" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jjh, resolution 2.50&Aring;" />
 
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'''E2 DNA-binding Domain from Bovine Papillomavirus Type 1'''<br />
 
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==Overview==
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==E2 DNA-binding Domain from Bovine Papillomavirus Type 1==
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The 2.5 A crystal structures of the DNA-binding domain of the E2 protein from bovine papillomavirus strain 1 and its complex with DNA are presented. E2 is a transcriptional regulatory protein that is also involved in viral DNA replication. It is the structural prototype for a novel class of DNA-binding proteins: dimeric beta-barrels with surface alpha-helices that serve as recognition helices. These helices contain the amino-acid residues involved in sequence-specifying interactions. The E2 proteins from different papillomavirus strains recognize and bind to the same consensus 12 base-pair DNA sequence. However, recent evidence from solution studies points to differences in the mechanisms by which E2 from the related viral strains bovine papillomavirus-1 and human papillomavirus-16 discriminate between DNA targets based on non-contacted nucleotide sequences. This report provides evidence that sequence-specific DNA-binding is accompanied by a rearrangement of protein subunits and deformation of the DNA. These results suggest that, along with DNA sequence-dependent conformational properties, protein subunit orientation plays a significant role in the mechanisms of target selection utilized by E2.
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<StructureSection load='1jjh' size='340' side='right'caption='[[1jjh]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jjh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Deltapapillomavirus_4 Deltapapillomavirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jjh OCA], [https://pdbe.org/1jjh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jjh RCSB], [https://www.ebi.ac.uk/pdbsum/1jjh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jjh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VE2_BPV1 VE2_BPV1] E2 regulates viral transcription and DNA replication. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/1jjh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jjh ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JJH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus_type_1 Bovine papillomavirus type 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJH OCA].
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*[[Regulatory protein E2|Regulatory protein E2]]
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__TOC__
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==Reference==
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</StructureSection>
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Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein., Hegde RS, Wang AF, Kim SS, Schapira M, J Mol Biol. 1998 Mar 6;276(4):797-808. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9500927 9500927]
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[[Category: Deltapapillomavirus 4]]
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[[Category: Bovine papillomavirus type 1]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Hegde RS]]
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[[Category: Hegde, R S.]]
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[[Category: Kim SS]]
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[[Category: Kim, S S.]]
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[[Category: Schapira M]]
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[[Category: Schapira, M.]]
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[[Category: Wang AF]]
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[[Category: Wang, A F.]]
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[[Category: bpv-1]]
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[[Category: dna-binding domain]]
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[[Category: e2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:24 2008''
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Current revision

E2 DNA-binding Domain from Bovine Papillomavirus Type 1

PDB ID 1jjh

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