1jny

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[[Image:1jny.jpg|left|200px]]<br /><applet load="1jny" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jny, resolution 1.8&Aring;" />
 
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'''Crystal structure of Sulfolobus solfataricus elongation factor 1 alpha in complex with GDP'''<br />
 
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==Overview==
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==Crystal structure of Sulfolobus solfataricus elongation factor 1 alpha in complex with GDP==
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The crystal structure of elongation factor 1alpha from the archaeon Sulfolobus solfataricus in complex with GDP (SsEF-1alpha.GDP) at 1.8 A resolution is reported. As already known for the eubacterial elongation factor Tu, the SsEF-1alpha.GDP structure consists of three different structural domains. Surprisingly, the analysis of the GDP-binding site reveals that the nucleotide- protein interactions are not mediated by Mg(2+). Furthermore, the residues that usually co-ordinate Mg(2+) through water molecules in the GTP-binding proteins, though conserved in SsEF-1alpha, are located quite far from the binding site. [(3)H]GDP binding experiments confirm that Mg(2+) has only a marginal effect on the nucleotide exchange reaction of SsEF-1alpha, although essential to GTPase activity elicited by SsEF-1alpha. Finally, structural comparisons of SsEF- 1alpha.GDP with yeast EF-1alpha in complex with the nucleotide exchange factor EF-1beta shows that a dramatic rearrangement of the overall structure of EF-1alpha occurs during the nucleotide exchange.
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<StructureSection load='1jny' size='340' side='right'caption='[[1jny]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jny]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JNY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jny OCA], [https://pdbe.org/1jny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jny RCSB], [https://www.ebi.ac.uk/pdbsum/1jny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jny ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EF1A_SACS2 EF1A_SACS2] This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/1jny_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jny ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JNY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNY OCA].
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of Sulfolobus solfataricus elongation factor 1alpha in complex with GDP reveals novel features in nucleotide binding and exchange., Vitagliano L, Masullo M, Sica F, Zagari A, Bocchini V, EMBO J. 2001 Oct 1;20(19):5305-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11574461 11574461]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Bocchini V]]
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[[Category: Bocchini, V.]]
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[[Category: Masullo M]]
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[[Category: Masullo, M.]]
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[[Category: Sica F]]
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[[Category: Sica, F.]]
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[[Category: Vitagliano L]]
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[[Category: Vitagliano, L.]]
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[[Category: Zagari A]]
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[[Category: Zagari, A.]]
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[[Category: GDP]]
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[[Category: alpha/beta structure]]
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[[Category: gtpase]]
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[[Category: protein biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:45 2008''
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Current revision

Crystal structure of Sulfolobus solfataricus elongation factor 1 alpha in complex with GDP

PDB ID 1jny

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