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1joc

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[[Image:1joc.gif|left|200px]]<br /><applet load="1joc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1joc, resolution 2.2&Aring;" />
 
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'''EEA1 homodimer of C-terminal FYVE domain bound to inositol 1,3-diphosphate'''<br />
 
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==Overview==
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==EEA1 homodimer of C-terminal FYVE domain bound to inositol 1,3-diphosphate==
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Early endosome autoantigen localization to early endosomes is mediated by a C-terminal region, which includes a calmodulin binding motif, a Rab5 interaction site, and a FYVE domain that selectively binds phosphatidyl inositol 3-phosphate. The crystal structure of the C-terminal region bound to inositol 1,3-bisphosphate reveals an organized, quaternary assembly consisting of a parallel coiled coil and a dyad-symmetric FYVE domain homodimer. Structural and biochemical observations support a multivalent mechanism for endosomal localization in which domain organization, dimerization, and quaternary structure amplify the weak affinity and modest specificity of head group interactions with conserved residues. A unique mode of membrane engagement deduced from the quaternary structure of the C-terminal region provides insight into the structural basis of endosome tethering.
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<StructureSection load='1joc' size='340' side='right'caption='[[1joc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1joc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The March 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Zinc Fingers'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_3 10.2210/rcsb_pdb/mom_2007_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JOC FirstGlance]. <br>
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Known disease associated with this structure: Spastic paraplegia 33 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=610243 610243]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ITP:PHOSPHORIC+ACID+MONO-(2,3,4,6-TETRAHYDROXY-5-PHOSPHONOOXY-CYCLOHEXYL)+ESTER'>ITP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1joc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1joc OCA], [https://pdbe.org/1joc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1joc RCSB], [https://www.ebi.ac.uk/pdbsum/1joc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1joc ProSAT]</span></td></tr>
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1JOC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=ITP:'>ITP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1JOC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb87_1.html Zinc Fingers]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOC OCA].
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</table>
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== Function ==
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==Reference==
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[https://www.uniprot.org/uniprot/EEA1_HUMAN EEA1_HUMAN] Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
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Multivalent endosome targeting by homodimeric EEA1., Dumas JJ, Merithew E, Sudharshan E, Rajamani D, Hayes S, Lawe D, Corvera S, Lambright DG, Mol Cell. 2001 Nov;8(5):947-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11741531 11741531]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jo/1joc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1joc ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Zinc Fingers]]
[[Category: Zinc Fingers]]
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[[Category: Corvera, S.]]
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[[Category: Corvera S]]
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[[Category: Dumas, J J.]]
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[[Category: Dumas JJ]]
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[[Category: Hayes, S.]]
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[[Category: Hayes S]]
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[[Category: Lambright, D G.]]
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[[Category: Lambright DG]]
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[[Category: Lawe, D.]]
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[[Category: Lawe D]]
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[[Category: Merithew, E.]]
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[[Category: Merithew E]]
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[[Category: Rajamani, D.]]
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[[Category: Rajamani D]]
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[[Category: ITP]]
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[[Category: ZN]]
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[[Category: fyve domain]]
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[[Category: inositol 3-phosphate binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:24:50 2008''
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Current revision

EEA1 homodimer of C-terminal FYVE domain bound to inositol 1,3-diphosphate

PDB ID 1joc

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