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(New page: == Your Heading Here (maybe something like 'Structure') == <StructureSection load='1dq8' size='350' side='right' caption='Structure of HMG-CoA reductase (PDB entry 1dq8)' scene=''> Any...) |
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| - | == | + | == Usage of bovine aconitase as a template for homology modelling of yeast aconitase == |
| - | <StructureSection load=' | + | <StructureSection load='1aco' size='350' side='right' caption='Structure bovine aconitase(PDB entry [[1aco]])' scene=''> |
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| + | Aconitase is an enzyme participating in the citric acid cycle. It is highly conserved among many species. | ||
| + | It has been shown <ref> pmid 15975908 </ref> that in yeast it is dually located in mitochondria and the cytosol by means of reverse translocation. To understand the underlying mechanism that enables this unique phenomena, a structural predicted model has been created using the I-TASSER homology modelling server <ref> pmid 18215316 </ref> <scene name='Sandbox_orly/Helices/1'>Bovine aconitase</scene> was used as the main template for this modelling. | ||
| + | Since prior experiments show that <scene name='Sandbox_orly/Yeast_six/2'>yeast aconitase's </scene> last six residues are crucial for its dual targeting <ref> PMID 21440554 </ref>, a structural comparison has been performed looking at the <scene name='Sandbox_orly/C-terminal_helix/2'>C-terminal helix </scene>. | ||
| + | It was found that an important <scene name='Sandbox_orly/Salt_bridge/2'> salt bridge </scene> exists in yeast aconitase where a <scene name='Sandbox_orly/Hydrophbic_pocket/4'>hydrophobic pocket</scene> exists instead in bovine aconitase ,in a very conserved area. This difference may be the underlying reason for the difference in the protein's behavior between different species. | ||
</StructureSection> | </StructureSection> | ||
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| + | <references/> | ||
Current revision
Usage of bovine aconitase as a template for homology modelling of yeast aconitase
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- ↑ Regev-Rudzki N, Karniely S, Ben-Haim NN, Pines O. Yeast aconitase in two locations and two metabolic pathways: seeing small amounts is believing. Mol Biol Cell. 2005 Sep;16(9):4163-71. Epub 2005 Jun 22. PMID:15975908 doi:10.1091/mbc.E04-11-1028
- ↑ Zhang Y. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics. 2008 Jan 23;9:40. PMID:18215316 doi:10.1186/1471-2105-9-40
- ↑ Ben-Menachem R, Regev-Rudzki N, Pines O. The aconitase C-terminal domain is an independent dual targeting element. J Mol Biol. 2011 Jun 3;409(2):113-23. Epub 2011 Apr 6. PMID:21440554 doi:10.1016/j.jmb.2011.03.045
