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1jqr

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[[Image:1jqr.jpg|left|200px]]<br /><applet load="1jqr" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jqr" />
 
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'''NMR structure of the African swine fever virus DNA polymerase X'''<br />
 
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==Overview==
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==NMR structure of the African swine fever virus DNA polymerase X==
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The African swine fever virus DNA polymerase X (ASFV Pol X or Pol X), the smallest known nucleotide polymerase, has recently been reported to be an extremely low fidelity polymerase that may be involved in strategic mutagenesis of the viral genome. Here we report the solution structure of Pol X. The structure, unique within the realm of nucleotide polymerases, consists of only palm and fingers subdomains. Despite the absence of a thumb subdomain, which is important for DNA binding in other polymerases, we show that Pol X binds DNA with very high affinity. Further structural analyses suggest a novel mode of DNA binding that may contribute to low fidelity synthesis. We also demonstrate that the ASFV DNA ligase is a low fidelity ligase capable of sealing a nick that contains a G-G mismatch. This supports the hypothesis of a virus-encoded, mutagenic base excision repair pathway consisting of a tandem Pol X/ligase mutator.
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<StructureSection load='1jqr' size='340' side='right'caption='[[1jqr]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jqr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/African_swine_fever_virus African swine fever virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JQR FirstGlance]. <br>
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1JQR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/African_swine_fever_virus African swine fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQR OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqr OCA], [https://pdbe.org/1jqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jqr RCSB], [https://www.ebi.ac.uk/pdbsum/1jqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jqr ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structure of a viral DNA polymerase X and evidence for a mutagenic function., Showalter AK, Byeon IJ, Su MI, Tsai MD, Nat Struct Biol. 2001 Nov;8(11):942-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11685239 11685239]
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== Function ==
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[https://www.uniprot.org/uniprot/DPOLX_ASFB7 DPOLX_ASFB7] Error-prone polymerase lacking a proofreading 3'-5' exonuclease which plays a role in viral DNA repair. Specifically binds intermediates in the single-nucleotide base-excision repair process. Also catalyzes DNA polymerization with low nucleotide-insertion fidelity. Together with the viral DNA ligase, fills the single nucleotide gaps generated by the AP endonuclease.<ref>PMID:12595253</ref> <ref>PMID:11685239</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/1jqr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jqr ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: African swine fever virus]]
[[Category: African swine fever virus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Byeon, I J.L.]]
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[[Category: Byeon I-JL]]
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[[Category: Showalter, A K.]]
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[[Category: Showalter AK]]
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[[Category: Su, M I.]]
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[[Category: Su M-I]]
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[[Category: Tsai, M D.]]
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[[Category: Tsai M-D]]
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[[Category: dna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:44 2008''
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Current revision

NMR structure of the African swine fever virus DNA polymerase X

PDB ID 1jqr

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