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1jsw

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[[Image:1jsw.gif|left|200px]]<br /><applet load="1jsw" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jsw, resolution 2.7&Aring;" />
 
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'''NATIVE L-ASPARTATE AMMONIA LYASE'''<br />
 
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==Overview==
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==NATIVE L-ASPARTATE AMMONIA LYASE==
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The X-ray crystal structure of l-aspartate ammonia-lyase has been determined to 2.8 A resolution. The enzyme contains three domains, and each domain is composed almost completely of alpha helices. The central domain is composed of five long helices. In the tetramer, these five helices form a 20-helix cluster. Such clusters have also been seen in delta-crystallin and in fumarase. The active site of aspartase has been located in a region that contains side chains from three different subunits. The structure of the apoenzyme has made it possible to identify some of the residues that are involved in binding the substrate. These residues have been examined by site-directed mutagenesis, and their putative roles have been assigned [Jayasekera, M. M. K., Shi, W., Farber, G. K., &amp; Viola, R. E. (1997) Biochemistry 36, 9145-9150].
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<StructureSection load='1jsw' size='340' side='right'caption='[[1jsw]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jsw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_B Escherichia coli B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JSW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JSW FirstGlance]. <br>
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1JSW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GLC:'>GLC</scene> and <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aspartate_ammonia-lyase Aspartate ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.1 4.3.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JSW OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jsw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jsw OCA], [https://pdbe.org/1jsw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jsw RCSB], [https://www.ebi.ac.uk/pdbsum/1jsw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jsw ProSAT]</span></td></tr>
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The structure of L-aspartate ammonia-lyase from Escherichia coli., Shi W, Dunbar J, Jayasekera MM, Viola RE, Farber GK, Biochemistry. 1997 Jul 29;36(30):9136-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9230045 9230045]
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</table>
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[[Category: Aspartate ammonia-lyase]]
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== Function ==
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[[Category: Escherichia coli]]
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[https://www.uniprot.org/uniprot/ASPA_ECOLI ASPA_ECOLI]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Dunbar, J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Farber, G K.]]
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Check<jmol>
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[[Category: Shi, W.]]
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<jmolCheckbox>
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[[Category: ACT]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/js/1jsw_consurf.spt"</scriptWhenChecked>
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[[Category: GLC]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: amino acid ammonia-lyase]]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:26:26 2008''
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jsw ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli B]]
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[[Category: Large Structures]]
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[[Category: Dunbar J]]
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[[Category: Farber GK]]
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[[Category: Shi W]]

Current revision

NATIVE L-ASPARTATE AMMONIA LYASE

PDB ID 1jsw

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