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4ec9

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[[Image:4ec9.jpg|left|200px]]
 
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{{STRUCTURE_4ec9| PDB=4ec9 | SCENE= }}
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==Crystal structure of full-length cdk9 in complex with cyclin t==
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<StructureSection load='4ec9' size='340' side='right'caption='[[4ec9]], [[Resolution|resolution]] 3.21&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ec9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EC9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ec9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ec9 OCA], [https://pdbe.org/4ec9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ec9 RCSB], [https://www.ebi.ac.uk/pdbsum/4ec9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ec9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/CCNT1_HUMAN CCNT1_HUMAN]] Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CDK9, the kinase of positive transcription elongation factor b (P-TEFb), stimulates transcription elongation by phosphorylating RNA polymerase II and transcription elongation factors. Using kinetic analysis of a human P-TEFb complex consisting of CDK9 and cyclin T, we show that the CDK9 C-terminal tail sequence is important for the catalytic mechanism and imposes an ordered binding of substrates and release of products. Crystallographic analysis of a CDK9/cyclin T complex in which the C-terminal tail partially blocks the ATP binding site reveals a possible reaction intermediate. Biochemical characterization of CDK9 mutants supports a model in which the CDK9 tail cycles through different conformational states. We propose that this mechanism is critical for the pattern of CTD Ser2 phosphorylation on actively transcribed genes.
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===Crystal structure of full-length cdk9 in complex with cyclin t===
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The CDK9 tail determines the reaction pathway of positive transcription elongation factor b.,Baumli S, Hole AJ, Wang LZ, Noble ME, Endicott JA Structure. 2012 Oct 10;20(10):1788-95. doi: 10.1016/j.str.2012.08.011. Epub 2012 , Sep 6. PMID:22959624<ref>PMID:22959624</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ec9" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[4ec9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EC9 OCA].
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*[[Cyclin 3D structures|Cyclin 3D structures]]
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*[[Cyclin-dependent kinase 3D structures|Cyclin-dependent kinase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Baumli, S.]]
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[[Category: Large Structures]]
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[[Category: Endicott, J A.]]
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[[Category: Baumli S]]
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[[Category: Hole, A J.]]
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[[Category: Endicott JA]]
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[[Category: Cyclin]]
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[[Category: Hole AJ]]
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[[Category: Cyclin dependent kinase]]
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[[Category: Kinase]]
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[[Category: Nuclear]]
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[[Category: Phosphorylation]]
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[[Category: Transferase-protein binding complex]]
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Current revision

Crystal structure of full-length cdk9 in complex with cyclin t

PDB ID 4ec9

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