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2j83

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[[Image:2j83.gif|left|200px]]<br />
 
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<applet load="2j83" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2j83, resolution 2.00&Aring;" />
 
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'''ULILYSIN METALLOPROTEASE IN COMPLEX WITH BATIMASTAT.'''<br />
 
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==Overview==
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==Ulilysin metalloprotease in complex with batimastat.==
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Human pappalysin-1 is a multi-domain metalloprotease engaged in the, homeostasis of insulin-like growth factors and the founding member of the, pappalysin family within the metzincin clan of metalloproteases. We have, recently identified an archaeal relative, ulilysin, encompassing only the, protease domain. It is a 262-residue active protease with a novel 3D, structure with two subdomains separated by an active-site cleft. Despite, negligible overall sequence similarity, noticeable similarity is found, with other metzincin prototypes, adamalysins/ADAMs and matrix, metalloproteinases. Ulilysin has been crystallised in a product complex, with an arginine-valine dipeptide occupying the active-site S(1') and, S(2') positions and in a complex with the broad-spectrum hydroxamic, acid-based ... [[http://ispc.weizmann.ac.il/pmbin/getpm?17097044 (full description)]]
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<StructureSection load='2j83' size='340' side='right'caption='[[2j83]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2j83]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_acetivorans_C2A Methanosarcina acetivorans C2A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J83 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J83 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BAT:4-(N-HYDROXYAMINO)-2R-ISOBUTYL-2S-(2-THIENYLTHIOMETHYL)SUCCINYL-L-PHENYLALANINE-N-METHYLAMIDE'>BAT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j83 OCA], [https://pdbe.org/2j83 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j83 RCSB], [https://www.ebi.ac.uk/pdbsum/2j83 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j83 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ULIL_METAC ULIL_METAC] Metalloprotease which in vitro specifically cleaves IGFBP-2 to -6, insulin, and extracellular matrix proteins but not IGFBP-1 or IGF-II. Shows a preference for substrates with an arginine in the P1' position, the first position downstream of the scissile bond.<ref>PMID:16627477</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j8/2j83_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j83 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human pappalysin-1 is a multi-domain metalloprotease engaged in the homeostasis of insulin-like growth factors and the founding member of the pappalysin family within the metzincin clan of metalloproteases. We have recently identified an archaeal relative, ulilysin, encompassing only the protease domain. It is a 262-residue active protease with a novel 3D structure with two subdomains separated by an active-site cleft. Despite negligible overall sequence similarity, noticeable similarity is found with other metzincin prototypes, adamalysins/ADAMs and matrix metalloproteinases. Ulilysin has been crystallised in a product complex with an arginine-valine dipeptide occupying the active-site S(1') and S(2') positions and in a complex with the broad-spectrum hydroxamic acid-based metalloprotease inhibitor, batimastat. This molecule inhibits mature ulilysin with an IC(50) value of 61 microM under the conditions assayed. The binding of batimastat to ulilysin evokes binding to vertebrate matrix metalloproteases but is much weaker. These data give insight into substrate specificity and mechanism of action and inhibition of the novel pappalysin family.
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==About this Structure==
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Substrate specificity of a metalloprotease of the pappalysin family revealed by an inhibitor and a product complex.,Garcia-Castellanos R, Tallant C, Marrero A, Sola M, Baumann U, Gomis-Ruth FX Arch Biochem Biophys. 2007 Jan 1;457(1):57-72. Epub 2006 Oct 24. PMID:17097044<ref>PMID:17097044</ref>
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2J83 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Methanosarcina_acetivorans Methanosarcina acetivorans]] with CA, ZN, BAT and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J83 OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Substrate specificity of a metalloprotease of the pappalysin family revealed by an inhibitor and a product complex., Garcia-Castellanos R, Tallant C, Marrero A, Sola M, Baumann U, Gomis-Ruth FX, Arch Biochem Biophys. 2007 Jan 1;457(1):57-72. Epub 2006 Oct 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17097044 17097044]
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</div>
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[[Category: Methanosarcina acetivorans]]
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<div class="pdbe-citations 2j83" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Baumann, U.]]
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<references/>
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[[Category: Garcia-Castellanos, R.]]
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__TOC__
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[[Category: Gomis-Ruth, F.X.]]
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</StructureSection>
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[[Category: Marrero, A.]]
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[[Category: Large Structures]]
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[[Category: Sola, M.]]
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[[Category: Methanosarcina acetivorans C2A]]
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[[Category: Tallant, C.]]
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[[Category: Baumann U]]
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[[Category: BAT]]
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[[Category: Garcia-Castellanos R]]
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[[Category: CA]]
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[[Category: Gomis-Ruth FX]]
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[[Category: GOL]]
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[[Category: Marrero A]]
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[[Category: ZN]]
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[[Category: Sola M]]
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[[Category: batimastat]]
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[[Category: Tallant C]]
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[[Category: bb-94]]
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[[Category: cancer]]
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[[Category: hydrolase]]
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[[Category: hydroxamate inhibitor]]
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[[Category: hypothetical protein]]
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[[Category: igf]]
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[[Category: igfbp protease]]
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[[Category: metalloprotease]]
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[[Category: metzincin]]
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[[Category: pappalysin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:28:18 2007''
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Current revision

Ulilysin metalloprotease in complex with batimastat.

PDB ID 2j83

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