This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1k0x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:56, 3 April 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1k0x.jpg|left|200px]]<br /><applet load="1k0x" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1k0x" />
 
-
'''Solution Structure of Melanoma Inhibitory Activity Protein'''<br />
 
-
==Overview==
+
==Solution Structure of Melanoma Inhibitory Activity Protein==
-
Melanoma inhibitory activity (MIA) is a small secreted protein that is implicated in cartilage cell maintenance and melanoma metastasis. It is representative of a recently discovered family of proteins that contain a Src Homologous 3 (SH3) subdomain. While SH3 domains are normally found in intracellular proteins and mediate protein-protein interactions via recognition of polyproline helices, MIA is single-domain extracellular protein, and it probably binds to a different class of ligands. Here we report the assignments, solution structure, and dynamics of human MIA determined by heteronuclear NMR methods. The structures were calculated in a semi-automated manner without manual assignment of NOE crosspeaks, and have a backbone rmsd of 0.38 A over the ordered regions of the protein. The structure consists of an SH3-like subdomain with N- and C-terminal extensions of approximately 20 amino acids each that together form a novel fold. The rmsd between the solution structure and our recently reported crystal structure is 0.86 A over the ordered regions of the backbone, and the main differences are localized to the most dynamic regions of the protein. The similarity between the NMR and crystal structures supports the use of automated NOE assignments and ambiguous restraints to accelerate the calculation of NMR structures.
+
<StructureSection load='1k0x' size='340' side='right'caption='[[1k0x]]' scene=''>
-
 
+
== Structural highlights ==
-
==Disease==
+
<table><tr><td colspan='2'>[[1k0x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0X FirstGlance]. <br>
-
Known disease associated with this structure: Myocardial infarction, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=611082 611082]]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0x OCA], [https://pdbe.org/1k0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0x RCSB], [https://www.ebi.ac.uk/pdbsum/1k0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0x ProSAT]</span></td></tr>
-
==About this Structure==
+
</table>
-
1K0X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0X OCA].
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/MIA_HUMAN MIA_HUMAN] Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
-
==Reference==
+
== Evolutionary Conservation ==
-
Solution structure and dynamics of melanoma inhibitory activity protein., Lougheed JC, Domaille PJ, Handel TM, J Biomol NMR. 2002 Mar;22(3):211-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11991352 11991352]
+
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0x_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0x ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Domaille, P J.]]
+
[[Category: Domaille PJ]]
-
[[Category: Handel, T M.]]
+
[[Category: Handel TM]]
-
[[Category: Lougheed, J C.]]
+
[[Category: Lougheed JC]]
-
[[Category: sh3 subdomain]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:28:55 2008''
+

Current revision

Solution Structure of Melanoma Inhibitory Activity Protein

PDB ID 1k0x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k0x"
Personal tools