1k66

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:44, 7 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1k66.jpg|left|200px]]<br /><applet load="1k66" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1k66, resolution 1.75&Aring;" />
 
-
'''Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB'''<br />
 
-
==Overview==
+
==Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB==
-
The structures of two response regulators (RRs) from the cyanobacterium Calothrix PCC7601, RcpA and RcpB, were solved to 1.9- and 1.75-A resolution, respectively. RcpA was found in phosphorylated and RcpB in nonphosphorylated form. Both RRs are members of phytochrome-associated, light-sensing two-component signal transduction pathways, based on histidine kinase-mediated receptor autophosphorylation and phosphorelay to a RR. Despite the overall folding similarity to CheY-type RRs ((beta/alpha)(5)-motif), RcpA and RcpB form homodimers, irrespective of their phosphorylation state, giving insight into a signal transduction putatively different from that of other known RRs. Dimerization is accomplished by a C-terminal extension of the RR polypeptide chain, and the surface formed by H4, beta 5, and H5, which constitute a hydrophobic contact area with distinct interactions between residues of either subunit. Sequence alignments reveal that the identified dimerization motif is archetypal for phytochrome-associated RRs, making them a novel subgroup of CheY-type RRs. The protein structures of RcpA and RcpB are compared to the recently presented protein structure of Rcp1 from Synechocystis.
+
<StructureSection load='1k66' size='340' side='right'caption='[[1k66]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1k66]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tolypothrix_sp._PCC_7601 Tolypothrix sp. PCC 7601]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K66 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k66 OCA], [https://pdbe.org/1k66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k66 RCSB], [https://www.ebi.ac.uk/pdbsum/1k66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k66 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q8RTM8_9CYAN Q8RTM8_9CYAN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k6/1k66_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k66 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1K66 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tolypothrix_sp._pcc_7601 Tolypothrix sp. pcc 7601] with <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K66 OCA].
+
*[[Response regulator 3D structure|Response regulator 3D structure]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structures of two cyanobacterial response regulators in apo- and phosphorylated form reveal a novel dimerization motif of phytochrome-associated response regulators., Benda C, Scheufler C, Tandeau de Marsac N, Gartner W, Biophys J. 2004 Jul;87(1):476-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15240481 15240481]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Tolypothrix sp. PCC 7601]]
-
[[Category: Tolypothrix sp. pcc 7601]]
+
[[Category: Benda C]]
-
[[Category: Benda, C.]]
+
[[Category: Gaertner W]]
-
[[Category: Gaertner, W.]]
+
[[Category: Scheufler C]]
-
[[Category: Marsac, N Tandeau de.]]
+
[[Category: Tandeau de Marsac N]]
-
[[Category: Scheufler, C.]]
+
-
[[Category: BME]]
+
-
[[Category: (beta/alpha)5]]
+
-
[[Category: apo-protein]]
+
-
[[Category: chey homologue]]
+
-
[[Category: homodimer]]
+
-
[[Category: response regulator]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:32 2008''
+

Current revision

Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB

PDB ID 1k66

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k66"
Personal tools