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1k6d

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[[Image:1k6d.gif|left|200px]]<br /><applet load="1k6d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k6d, resolution 1.90&Aring;" />
 
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'''CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT==
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The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (&gt;3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.
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<StructureSection load='1k6d' size='340' side='right'caption='[[1k6d]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1k6d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K6D FirstGlance]. <br>
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1K6D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetate_CoA-transferase Acetate CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.8 2.8.3.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k6d OCA], [https://pdbe.org/1k6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k6d RCSB], [https://www.ebi.ac.uk/pdbsum/1k6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k6d ProSAT], [https://www.topsan.org/Proteins/MCSG/1k6d TOPSAN]</span></td></tr>
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Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data., Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. Epub 2002, Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12454473 12454473]
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</table>
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[[Category: Acetate CoA-transferase]]
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== Function ==
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[https://www.uniprot.org/uniprot/ATOD_ECOLI ATOD_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k6/1k6d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k6d ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Collart, F.]]
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[[Category: Collart F]]
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[[Category: Dementieva, I.]]
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[[Category: Dementieva I]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: Korolev, S.]]
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[[Category: Korolev S]]
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[[Category: Koroleva, O.]]
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[[Category: Koroleva O]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Petterson K]]
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[[Category: Petterson, K.]]
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[[Category: MG]]
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[[Category: mcsg]]
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[[Category: midwest center for structural genomics]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:36 2008''
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Current revision

CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT

PDB ID 1k6d

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