1k86

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[[Image:1k86.gif|left|200px]]<br /><applet load="1k86" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1k86, resolution 2.6&Aring;" />
 
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'''Crystal structure of caspase-7'''<br />
 
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==Overview==
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==Crystal structure of caspase-7==
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Apoptosis is primarily executed by active caspases, which are derived from the inactive procaspase zymogens through proteolytic cleavage. Here we report the crystal structures of a caspase zymogen, procaspase-7, and an active caspase-7 without any bound inhibitors. Compared to the inhibitor-bound caspase-7, procaspase-7 zymogen exhibits significant structural differences surrounding the catalytic cleft, which precludes the formation of a productive conformation. Proteolytic cleavage between the large and small subunits allows rearrangement of essential loops in the active site, priming active caspase-7 for inhibitor/substrate binding. Strikingly, binding by inhibitors causes a 180 degrees flipping of the N terminus in the small subunit, which interacts with and stabilizes the catalytic cleft. These analyses reveal the structural mechanisms of caspase activation and demonstrate that the inhibitor/substrate binding is a process of induced fit.
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<StructureSection load='1k86' size='340' side='right'caption='[[1k86]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k86]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K86 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k86 OCA], [https://pdbe.org/1k86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k86 RCSB], [https://www.ebi.ac.uk/pdbsum/1k86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k86 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CASP7_HUMAN CASP7_HUMAN] Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/1k86_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k86 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1K86 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K86 OCA].
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*[[Caspase 3D structures|Caspase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of a procaspase-7 zymogen: mechanisms of activation and substrate binding., Chai J, Wu Q, Shiozaki E, Srinivasula SM, Alnemri ES, Shi Y, Cell. 2001 Nov 2;107(3):399-407. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11701129 11701129]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alnemri, E S.]]
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[[Category: Alnemri ES]]
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[[Category: Chai, J.]]
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[[Category: Chai J]]
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[[Category: Shi, Y.]]
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[[Category: Shi Y]]
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[[Category: Shiozaki, E.]]
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[[Category: Shiozaki E]]
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[[Category: Srinivasa, S M.]]
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[[Category: Srinivasa SM]]
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[[Category: Wu, Q.]]
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[[Category: Wu Q]]
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[[Category: activation]]
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[[Category: apoptosis]]
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[[Category: caspase]]
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[[Category: zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:31:09 2008''
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Current revision

Crystal structure of caspase-7

PDB ID 1k86

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