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1kaq

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[[Image:1kaq.gif|left|200px]]<br /><applet load="1kaq" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kaq, resolution 3.20&Aring;" />
 
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'''Structure of Bacillus subtilis Nicotinic Acid Mononucleotide Adenylyl Transferase'''<br />
 
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==Overview==
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==Structure of Bacillus subtilis Nicotinic Acid Mononucleotide Adenylyl Transferase==
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The nadD gene, encoding the enzyme nicotinic acid mononucleotide (NaMN) adenylyltransferase (AT), is essential for the synthesis of NAD and subsequent viability of the cell. The nadD gene in Bacillus subtilis (yqeJ) was identified by sequence homology with other bacterial nadD genes and by biochemical characterization of the gene product. NaMN AT catalyzes the reversible adenylation of both NaMN and the nicotinamide mononucleotide (NMN) but shows specificity for the nicotinate. In contrast to other known NMN ATs, biophysical characterizations reveal it to be a dimer. The NaMN AT crystal structure was determined for both the apo enzyme and product-bound form, to 2.1 and 3.2 A, respectively. The structures reveal a "functional" dimer conserved in both crystal forms and a monomer fold common to members of the nucleotidyl-transferase alpha/beta phosphodiesterase superfamily. A structural comparison with family members suggests a new conserved motif (SXXXX(R/K)) at the N terminus of an alpha-helix, which is not part of the shared fold. Interactions of the nicotinic acid with backbone atoms indicate the structural basis for specificity.
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<StructureSection load='1kaq' size='340' side='right'caption='[[1kaq]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1kaq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KAQ FirstGlance]. <br>
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1KAQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=DND:'>DND</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nicotinate-nucleotide_adenylyltransferase Nicotinate-nucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.18 2.7.7.18] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DND:NICOTINIC+ACID+ADENINE+DINUCLEOTIDE'>DND</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kaq OCA], [https://pdbe.org/1kaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kaq RCSB], [https://www.ebi.ac.uk/pdbsum/1kaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kaq ProSAT]</span></td></tr>
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Identification, characterization, and crystal structure of Bacillus subtilis nicotinic acid mononucleotide adenylyltransferase., Olland AM, Underwood KW, Czerwinski RM, Lo MC, Aulabaugh A, Bard J, Stahl ML, Somers WS, Sullivan FX, Chopra R, J Biol Chem. 2002 Feb 1;277(5):3698-707. Epub 2001 Nov 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11704676 11704676]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NADD_BACSU NADD_BACSU] Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ka/1kaq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kaq ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Nicotinate-nucleotide adenylyltransferase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Aulabaugh A]]
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[[Category: Aulabaugh, A.]]
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[[Category: Bard J]]
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[[Category: Bard, J.]]
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[[Category: Chopra R]]
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[[Category: Chopra, R.]]
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[[Category: Czerwinski RM]]
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[[Category: Czerwinski, R M.]]
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[[Category: Lo MC]]
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[[Category: Lo, M C.]]
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[[Category: Olland AM]]
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[[Category: Olland, A M.]]
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[[Category: Somers WS]]
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[[Category: Somers, W S.]]
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[[Category: Stahl ML]]
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[[Category: Stahl, M L.]]
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[[Category: Sullivan FX]]
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[[Category: Sullivan, F X.]]
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[[Category: Underwood KW]]
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[[Category: Underwood, K W.]]
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[[Category: DND]]
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[[Category: naad]]
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[[Category: rossman fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:31:58 2008''
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Current revision

Structure of Bacillus subtilis Nicotinic Acid Mononucleotide Adenylyl Transferase

PDB ID 1kaq

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