1kdl

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[[Image:1kdl.gif|left|200px]]<br /><applet load="1kdl" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kdl" />
 
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'''Solution structure of the amphipathic domain of YopD from Yersinia'''<br />
 
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==Overview==
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==Solution structure of the amphipathic domain of YopD from Yersinia==
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To establish an infection, Yersinia pseudotuberculosis utilizes a plasmid-encoded type III secretion machine that permits the translocation of several anti-host factors into the cytosol of target eukaryotic cells. Secreted YopD is essential for this process. Pre-secretory stabilization of YopD is mediated by an interaction with its cognate chaperone, LcrH. YopD possesses LcrH binding domains located in the N-terminus and in a predicted amphipathic domain located near the C-terminus. This latter domain is also critical for Yersinia virulence. In this study, we designed synthetic peptides encompassing the C-terminal amphipathic domain of YopD. A solution structure of YopD278-300, a peptide that strongly interacted with LcrH, was obtained by NMR methods. The structure is composed of a well-defined amphipathic alpha helix ranging from Phe280 to Tyr291, followed by a type I beta turn between residues Val292 and His295. The C-terminal truncated peptides, YopD278-292 and YopD271-292, lacked helical structure, implicating the beta turn in helix stability. An interaction between YopD278-300 and its cognate chaperone, LcrH, was observed by NMR through line-broadening effects and chemical shift differences between the free peptide and the peptide-LcrH complex. These effects were not observed for the unstructured peptide, YopD278-292, which confirms that the alpha helical structure of the YopD amphipathic domain is a critical binding region of LcrH.
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<StructureSection load='1kdl' size='340' side='right'caption='[[1kdl]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1kdl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis_IP_32953 Yersinia pseudotuberculosis IP 32953]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KDL FirstGlance]. <br>
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1KDL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KDL OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kdl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kdl OCA], [https://pdbe.org/1kdl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kdl RCSB], [https://www.ebi.ac.uk/pdbsum/1kdl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kdl ProSAT]</span></td></tr>
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==Reference==
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</table>
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Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia., Tengel T, Sethson I, Francis MS, Eur J Biochem. 2002 Aug;269(15):3659-68. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12153562 12153562]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/YOPD_YERPS YOPD_YERPS] Interacts with the target membrane to form channel structures involved either in translocation of YopE into the cytoplasmic space of the target cell or in the cytotoxic effect of the YopE protein.
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[[Category: Francis, M S.]]
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__TOC__
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[[Category: Sethson, I.]]
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</StructureSection>
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[[Category: Tengel, T.]]
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[[Category: Large Structures]]
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[[Category: amphipathic alpha helix]]
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[[Category: Yersinia pseudotuberculosis IP 32953]]
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[[Category: beta turn]]
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[[Category: Francis MS]]
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[[Category: yersinia]]
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[[Category: Sethson I]]
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[[Category: yopd]]
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[[Category: Tengel T]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:32:55 2008''
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Current revision

Solution structure of the amphipathic domain of YopD from Yersinia

PDB ID 1kdl

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