2xec

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:29, 20 December 2023) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2xec.png|left|200px]]
 
-
{{STRUCTURE_2xec| PDB=2xec | SCENE= }}
+
==Nocardia farcinica maleate cis-trans isomerase bound to TRIS==
 +
<StructureSection load='2xec' size='340' side='right'caption='[[2xec]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2xec]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Nocardia_farcinica_IFM_10152 Nocardia farcinica IFM 10152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XEC FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xec OCA], [https://pdbe.org/2xec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xec RCSB], [https://www.ebi.ac.uk/pdbsum/2xec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xec ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MAIA_NOCFA MAIA_NOCFA] Catalyzes cis-trans isomerization of the C2-C3 double bond in maleate to yield fumarate. Shows a strict specificity for maleate, with no activity detected toward structurally related substrates including citraconate, mesaconate, dimethylmaleate, and maleamide.<ref>PMID:20677745</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xe/2xec_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xec ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Maleate isomerase (MI), a member of the Asp/Glu racemase superfamily, catalyzes cis-trans isomerization of the C2-C3 double bond in maleate to yield fumarate. Mutational studies, in conjunction with the structure of the C194A mutant of Nocardia farcinica MI cocrystallized with maleate, have revealed an unprecedented mode of catalysis for the superfamily in which the isomerization reaction is initiated by nucleophilic attack of cysteine at the double bond, yielding a covalent succinylcysteine-like intermediate.
-
===Nocardia farcinica maleate cis-trans isomerase bound to TRIS===
+
A Covalent Succinylcysteine-like Intermediate in the Enzyme-Catalyzed Transformation of Maleate to Fumarate by Maleate Isomerase.,Fisch F, Fleites CM, Delenne M, Baudendistel N, Hauer B, Turkenburg JP, Hart S, Bruce NC, Grogan G J Am Chem Soc. 2010 Aug 2. PMID:20677745<ref>PMID:20677745</ref>
-
{{ABSTRACT_PUBMED_20677745}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2xec" style="background-color:#fffaf0;"></div>
-
[[2xec]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Nocardia_farcinica Nocardia farcinica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XEC OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:020677745</ref><references group="xtra"/>
+
</StructureSection>
-
[[Category: Maleate isomerase]]
+
[[Category: Large Structures]]
-
[[Category: Nocardia farcinica]]
+
[[Category: Nocardia farcinica IFM 10152]]
-
[[Category: Baudendistel, N.]]
+
[[Category: Baudendistel N]]
-
[[Category: Bruce, N C.]]
+
[[Category: Bruce NC]]
-
[[Category: Fisch, F.]]
+
[[Category: Fisch F]]
-
[[Category: Grogan, G.]]
+
[[Category: Grogan G]]
-
[[Category: Hart, S.]]
+
[[Category: Hart S]]
-
[[Category: Hauer, B.]]
+
[[Category: Hauer B]]
-
[[Category: Martinez-Fleites, C.]]
+
[[Category: Martinez-Fleites C]]
-
[[Category: Turkenburg, J P.]]
+
[[Category: Turkenburg JP]]
-
[[Category: Isomerase]]
+

Current revision

Nocardia farcinica maleate cis-trans isomerase bound to TRIS

PDB ID 2xec

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xec"
Personal tools