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1kig

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[[Image:1kig.jpg|left|200px]]<br /><applet load="1kig" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kig, resolution 3.0&Aring;" />
 
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'''BOVINE FACTOR XA'''<br />
 
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==Overview==
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==BOVINE FACTOR XA==
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The structure of recombinant tick anticoagulant peptide (rTAP) complexed to bovine factor Xa at 3.0 A resolution reveals the structural basis for the specificity and the high affinity of rTAP. Three N-terminal residues, Tyr501, Asn502 and Arg503, play a critical role in the complex formation as suggested by earlier mutagenic studies and the ornithodorin-thrombin complex. Unexpectedly, the side-chain of Tyr501 is located in the S1 pocket, although factor Xa favors arginine as a P1 residue. Arg503 is located at the aryl binding pocket and forms a salt-bridge with Glu97 of factor Xa. The autolysis loop, which is disordered in the uninhibited factor Xa structure, is involved in the formation of the complex as a part of the secondary binding site. The C-terminal helix of rTAP interacts with factor Xa as a secondary binding determinant. The N-terminal residues of rTAP reorganize during the formation of the factor Xa-rTAP complex from the conformation found in the solution into an extended conformation. The presence of the secondary binding site confirms the proposed two-step kinetic mechanism based on the results of a mutagenesis study.
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<StructureSection load='1kig' size='340' side='right'caption='[[1kig]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kig]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Ornithodoros_moubata Ornithodoros moubata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KIG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kig OCA], [https://pdbe.org/1kig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kig RCSB], [https://www.ebi.ac.uk/pdbsum/1kig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kig ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FA10_BOVIN FA10_BOVIN] Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/1kig_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kig ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1KIG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Ornithodoros_moubata Ornithodoros moubata]. Active as [http://en.wikipedia.org/wiki/Coagulation_factor_Xa Coagulation factor Xa], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.6 3.4.21.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIG OCA].
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*[[Factor Xa|Factor Xa]]
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__TOC__
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==Reference==
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</StructureSection>
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Unexpected binding mode of tick anticoagulant peptide complexed to bovine factor Xa., Wei A, Alexander RS, Duke J, Ross H, Rosenfeld SA, Chang CH, J Mol Biol. 1998;283(1):147-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9761680 9761680]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Coagulation factor Xa]]
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[[Category: Large Structures]]
[[Category: Ornithodoros moubata]]
[[Category: Ornithodoros moubata]]
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[[Category: Protein complex]]
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[[Category: Alexander R]]
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[[Category: Alexander, R.]]
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[[Category: Chang C-H]]
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[[Category: Chang, C H.]]
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[[Category: Wei A]]
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[[Category: Wei, A.]]
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[[Category: blood coagulation]]
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[[Category: complex (protease/inhibitor)]]
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[[Category: glycoprotein]]
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[[Category: plasma]]
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[[Category: serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:34:34 2008''
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Current revision

BOVINE FACTOR XA

PDB ID 1kig

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