2ca0

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[[Image:2ca0.gif|left|200px]]<br />
 
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<applet load="2ca0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ca0, resolution 2.85&Aring;" />
 
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'''CRYSTAL STRUCTURE OF YC-17-BOUND CYTOCHROME P450 PIKC (CYP107L1)'''<br />
 
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==About this Structure==
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==Crystal structure of YC-17-bound cytochrome P450 PikC (CYP107L1)==
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2CA0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae]] with HEM and PXI as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CA0 OCA]].
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<StructureSection load='2ca0' size='340' side='right'caption='[[2ca0]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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[[Category: Single protein]]
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== Structural highlights ==
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[[Category: Streptomyces venezuelae]]
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<table><tr><td colspan='2'>[[2ca0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CA0 FirstGlance]. <br>
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[[Category: Kim, Y.]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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[[Category: Podust, L.M.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PXI:4-{[4-(DIMETHYLAMINO)-3-HYDROXY-6-METHYLTETRAHYDRO-2H-PYRAN-2-YL]OXY}-12-ETHYL-3,5,7,11-TETRAMETHYLOXACYCLODODEC-9-ENE-2,8-DIONE'>PXI</scene></td></tr>
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[[Category: Sherman, D.H.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ca0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca0 OCA], [https://pdbe.org/2ca0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ca0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ca0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ca0 ProSAT]</span></td></tr>
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[[Category: Waterman, M.R.]]
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</table>
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[[Category: Yermalitskaya, L.I.]]
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== Function ==
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[[Category: HEM]]
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[https://www.uniprot.org/uniprot/PIKC_STRVZ PIKC_STRVZ] Catalyzes the hydroxylation of narbomycin to give rise to pikromycin, and of 10-deoxymethymycin (YC-17) to give rise to methymycin and neomethymycin during macrolide antibiotic biosynthesis. In addition, produces low amounts of neopicromycin, novapikromycin and novamethymycin. Requires the participation of a ferredoxin and a ferredoxin reductase for the transfer of electrons from NADPH to the monooxygenase.<ref>PMID:16825192</ref> <ref>PMID:19124459</ref> <ref>PMID:19833867</ref> <ref>PMID:24627965</ref> <ref>PMID:9778370</ref> <ref>PMID:9831532</ref>
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[[Category: PXI]]
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== Evolutionary Conservation ==
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[[Category: antibiotic biosynthesis]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: cytochrome p450]]
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Check<jmol>
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[[Category: heme]]
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<jmolCheckbox>
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[[Category: iron]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ca/2ca0_consurf.spt"</scriptWhenChecked>
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[[Category: macrolide monooxygenase]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: metal-binding]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: oxidoreductase]]
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</jmolCheckbox>
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[[Category: pikc]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ca0 ConSurf].
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[[Category: yc-17]]
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<div style="clear:both"></div>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:31:50 2007''
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==See Also==
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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*[[Cytochrome P450 hydroxylase 3D structures|Cytochrome P450 hydroxylase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptomyces venezuelae]]
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[[Category: Kim Y]]
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[[Category: Podust LM]]
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[[Category: Sherman DH]]
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[[Category: Waterman MR]]
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[[Category: Yermalitskaya LI]]

Current revision

Crystal structure of YC-17-bound cytochrome P450 PikC (CYP107L1)

PDB ID 2ca0

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