1kmp

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[[Image:1kmp.jpg|left|200px]]<br /><applet load="1kmp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1kmp, resolution 2.50&Aring;" />
 
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'''Crystal structure of the Outer Membrane Transporter FecA Complexed with Ferric Citrate'''<br />
 
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==Overview==
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==Crystal structure of the Outer Membrane Transporter FecA Complexed with Ferric Citrate==
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Siderophore-mediated acquisition systems facilitate iron uptake. We present the crystallographic structure of the integral outer membrane receptor FecA from Escherichia coli with and without ferric citrate at 2.5 and 2.0 angstrom resolution. FecA is composed of three distinct domains: the barrel, plug, and NH2-terminal extension. Binding of ferric citrate triggers a conformational change of the extracellular loops that close the external pocket of FecA. Ligand-induced allosteric transitions are propagated through the outer membrane by the plug domain, signaling the occupancy of the receptor in the periplasm. These data establish the structural basis of gating for receptors dependent on the cytoplasmic membrane protein TonB. By compiling available data for this family of receptors, we propose a mechanism for the energy-dependent transport of siderophores.
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<StructureSection load='1kmp' size='340' side='right'caption='[[1kmp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1kmp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KMP FirstGlance]. <br>
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1KMP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=FDC:'>FDC</scene> and <scene name='pdbligand=LDA:'>LDA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KMP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kmp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kmp OCA], [https://pdbe.org/1kmp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kmp RCSB], [https://www.ebi.ac.uk/pdbsum/1kmp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kmp ProSAT]</span></td></tr>
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Structural basis of gating by the outer membrane transporter FecA., Ferguson AD, Chakraborty R, Smith BS, Esser L, van der Helm D, Deisenhofer J, Science. 2002 Mar 1;295(5560):1715-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11872840 11872840]
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</table>
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[[Category: Escherichia coli]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/FECA_ECOLI FECA_ECOLI] FecA is the outer membrane receptor protein in the Fe(3+) dicitrate transport system.
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[[Category: Chakraborty, R.]]
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== Evolutionary Conservation ==
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[[Category: Deisenhofer, J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Esser, L.]]
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Check<jmol>
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[[Category: Ferguson, A D.]]
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<jmolCheckbox>
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[[Category: Helm, D van der.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/km/1kmp_consurf.spt"</scriptWhenChecked>
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[[Category: Smith, B S.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: FDC]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: LDA]]
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</jmolCheckbox>
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[[Category: integral outer membrane protein]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kmp ConSurf].
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[[Category: iron]]
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<div style="clear:both"></div>
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[[Category: siderophore]]
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__TOC__
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[[Category: tonb-dependent receptor]]
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:35:47 2008''
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[[Category: Large Structures]]
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[[Category: Chakraborty R]]
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[[Category: Deisenhofer J]]
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[[Category: Esser L]]
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[[Category: Ferguson AD]]
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[[Category: Smith BS]]
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[[Category: Van der Helm D]]

Current revision

Crystal structure of the Outer Membrane Transporter FecA Complexed with Ferric Citrate

PDB ID 1kmp

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