1koc
From Proteopedia
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| - | [[Image:1koc.jpg|left|200px]]<br /><applet load="1koc" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1koc" /> | ||
| - | '''RNA APTAMER COMPLEXED WITH ARGININE, NMR'''<br /> | ||
| - | == | + | ==RNA APTAMER COMPLEXED WITH ARGININE, NMR== |
| + | <StructureSection load='1koc' size='340' side='right'caption='[[1koc]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1koc]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KOC FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1koc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1koc OCA], [https://pdbe.org/1koc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1koc RCSB], [https://www.ebi.ac.uk/pdbsum/1koc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1koc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
In a previous study, an RNA aptamer for the specific recognition of arginine was evolved from a parent sequence that bound citrulline specifically. The two RNAs differ at only 3 positions out of 44. The solution structures of the two aptamers complexed to their cognate amino acids have now been determined by two-dimensional nuclear magnetic resonance spectroscopy. Both aptamers contain two asymmetrical internal loops that are not well ordered in the free RNA but that fold into a compact structure upon ligand binding. Those nucleotides common to both RNAs include a conserved cluster of purine residues, three of which form an uneven plane containing a G:G pair, and two other residues nearly perpendicular to that surface. Two of the three variant nucleotides are stacked on the cluster of purines and form a triple contact to the amino acid side chain, whereas the edge of the third variant nucleotide is capping the binding pocket. | In a previous study, an RNA aptamer for the specific recognition of arginine was evolved from a parent sequence that bound citrulline specifically. The two RNAs differ at only 3 positions out of 44. The solution structures of the two aptamers complexed to their cognate amino acids have now been determined by two-dimensional nuclear magnetic resonance spectroscopy. Both aptamers contain two asymmetrical internal loops that are not well ordered in the free RNA but that fold into a compact structure upon ligand binding. Those nucleotides common to both RNAs include a conserved cluster of purine residues, three of which form an uneven plane containing a G:G pair, and two other residues nearly perpendicular to that surface. Two of the three variant nucleotides are stacked on the cluster of purines and form a triple contact to the amino acid side chain, whereas the edge of the third variant nucleotide is capping the binding pocket. | ||
| - | + | Structural basis of ligand discrimination by two related RNA aptamers resolved by NMR spectroscopy.,Yang Y, Kochoyan M, Burgstaller P, Westhof E, Famulok M Science. 1996 May 31;272(5266):1343-7. PMID:8650546<ref>PMID:8650546</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 1koc" style="background-color:#fffaf0;"></div> |
| - | [[Category: Burgstaller | + | == References == |
| - | [[Category: Famulok | + | <references/> |
| - | [[Category: Kochoyan | + | __TOC__ |
| - | [[Category: Westhof | + | </StructureSection> |
| - | [[Category: Yang | + | [[Category: Large Structures]] |
| - | + | [[Category: Burgstaller P]] | |
| - | + | [[Category: Famulok M]] | |
| - | + | [[Category: Kochoyan M]] | |
| - | + | [[Category: Westhof E]] | |
| - | + | [[Category: Yang YS]] | |
| - | + | ||
Current revision
RNA APTAMER COMPLEXED WITH ARGININE, NMR
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